Publication:
The Lian-Dou-Qing-Mai formula activates the PPARγ-LXRα-ABCA1/ABCG1 pathway by regulating IL-10, leading to the promotion of cholesterol efflux and a reduction in atherosclerotic plaques

Thumbnail Image
Files
Liao-40-585-596-2025.pdf(5.14 MB)
Date
2025
relationships.isAuthorOfPublication
relationships.isSecondaryAuthorOf
relationships.isDirectorOf
Authors
Liao, Wenqi ; Li, You ; Zhao, Haoyan ; Lu, Shu
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
publication.page.editor
publication.page.department
DOI
https://doi.org/10.14670/HH-18-803
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Background. To observe the effect of the Lian-Dou-Qing-Mai (LDQM) formula on lipid metabolism in mice and explore its mechanism from the perspective of regulating the PPARγ/LXRα/ABCA1 signaling pathway. Methods. THP-1 cells were induced to transform into foam cells with ox-LDL. Atherosclerosis (AS) models were constructed using a high-fat diet in ApoE-/- mice. Detection kits were used to evaluate triglyceride (TG) and total cholesterol (TC) content; TNF-α, MCP-1, MMP-9, TMP-1, PPARγ, LXRα, ABCA1, and ABCG1 mRNA and protein expression were identified using real-time PCR and western blot. Aortic plaque development and lipid deposition were seen using hematoxylin and eosin (HE) and oil red O staining, respectively. Results. In the cell model, LDQM could inhibit the formation of THP-1 macrophage-derived foam cells and the expression of inflammatory factors, promote macrophage cholesterol efflux, increase the expression of IL-10, and activate the PPARγ-LXRα-ABCA1/ ABCG1 pathway. Additional IL-10 treatment further promotes LDQM-induced cholesterol efflux in THP-1 cells. In in vivo models, LDQM inhibited the area of atherosclerotic lesions, aortic lipid deposition, and inflammation levels in ApoE-/- mice through IL-10, and activated the expression level of the PPARγ-LXRα-ABCA1/ABCG1 pathway. Conclusion. LDQM may affect the PPARγ/LXRα/ ABCA1 signaling pathway through IL-10, regulate lipid metabolism, reduce serum inflammatory expression and lipid deposition, and improve the formation of atheroplaques.
publication.page.subject
Lian Dou Qing Mai , PPARγ-LXRα-ABCA1/ABCG1 , IL-10 , Atherosclerosis
Citation
Histology and Histopathology Vol. 40, nº04 (2025)
URI
http://hdl.handle.net/10201/151915
item.page.embargo
Collections
Histology and histopathology Vol.40, nº4 (2025)
Ir a Estadísticas