Publication: Cytokeratin expression profiles of canine epithelial tissues
Authors
Rickmeyer, Tina ; Jäger, Kathrin ; Schöniger, Sandra ; Schoon, H.-A.
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-18-070
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info:eu-repo/semantics/article
Description
Abstract
Cytokeratins (CKs) are intermediate
filaments of epithelial cells. In humans, different types
of epithelia as well as their neoplasms show distinct CK
expression profiles.
The aim of this study was to establish a panel of
CKs for the identification of specialized canine epithelia
that can be integrated in a routine diagnostic setting.
Immunohistochemistry was performed on 42 formalinfixed paraffin-embedded (FFPE) canine unaltered tissues
including all epithelial tissues by using an antibody
panel detecting CKs 7, 8, 13, 14, 17, 19 and 20 and the
pancytokeratin marker AE1/AE3.
Using this antibody panel, a differentiation scheme
for the identification of canine tissues was developed.
This allowed the identification of 23 out of the 42
examined canine tissues and the distinction of 9 groups
of specialized epithelia. The statistical validation
revealed high variations in the immunoreactivity for
CKs 7, 8, 14, 17 and 20 between the donor dogs. The
antibody detecting CK 7 (OV-TL 12/13) showed a
decrease in immunostaining after a fixation time of 3
and 4 days.
To the best of the authors’ knowledge this is the first
study that characterizes all canine epithelial tissues for
their expression of CKs 7, 8, 13, 14, 17, 19 and 20 and
the pancytokeratin marker AE1/AE3. Results of this
study are an important prerequisite for comparative
histology and for the investigation into
similarities/differences of the cytokeratin expression
between normal and neoplastic epithelia. Since this
study was performed on FFPE tissue, it can be included
in the workflow of a routine diagnostic laboratory.
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Citation
Histology and Histopathology, Vol.34, nº6, (2019)
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