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Anti-leukemic activity of Brassica-derived bioactive compounds in HL-60 myeloid leukemia cells

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dc.contributor.authorNúñez-Sánchez, María Ángeles
dc.contributor.authorMartínez-Sánchez, María Antonia
dc.contributor.authorVerdejo-Sánchez, Marina
dc.contributor.authorGarcía-Ibáñez, Paula
dc.contributor.authorOliva Bolarín, Alba
dc.contributor.authorRamos-Molina, Bruno
dc.contributor.authorMoreno, Diego A.
dc.contributor.authorRuiz Alcaraz, Antonio José
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.contributor.otherFacultad de Biología
dc.date.accessioned2026-01-14T09:09:10Z
dc.date.available2026-01-14T09:09:10Z
dc.date.copyright© 2022 by the authors.
dc.date.issued2022-11-02
dc.description.abstractAcute myeloid leukemia (AML) is a cancer of the myeloid blood cells mainly treated with chemotherapy for cancer remission, but this non-selective treatment also induces numerous side effects. Investigations with bioactive compounds from plant-derived foods against cancer have increased in the last years because there is an urgent need to search for new anti-leukemic agents possessing higher efficacy and selectivity for AML cells and fewer negative side effects. In this study, we analyzed the anti-leukemic activity of several phytochemicals that are representative of the major classes of compounds present in cruciferous foods (glucosinolates, isothiocyanates, hydroxycinnamic acids, flavonols, and anthocyanins) in the human acute myeloid leukemia cell line HL-60. Our results revealed that among the different Brassica-derived compounds assayed, sulforaphane (SFN) (an aliphatic isothiocyanate) showed the most potent anti-leukemic activity with an IC50 value of 6 µM in dose-response MTT assays after 48 h of treatment. On the other hand, chlorogenic acid (a hydroxycinnamic acid) and cyanidin-3-glucoside (an anthocyanin) also displayed anti-leukemic potential, with IC50 values of 7 µM and 17 µM after 48 h of incubation, respectively. Importantly, these compounds did not show significant cell toxicity in macrophages-like differentiated cells at 10 and 25 µM, indicating that their cytotoxic effects were specific to AML cancer cells. Finally, we found that these three compounds were able to induce the NRF2/KEAP1 signaling pathway in a dose-dependent manner, highlighting SFN as the most potent NRF2 activator. Overall, the present evidence shed light on the potential for using foods and ingredients rich in anticancer bioactive phytochemicals from Brassica spp.
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dc.format.extent12
dc.identifier.citationInt. J. Mol. Sci. 2022, 23, 13400
dc.identifier.doihttps://doi.org/10.3390/ijms232113400
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10201/186689
dc.languageeng
dc.publisherMDPI
dc.relationThis research was funded by Fundación Séneca–Murcia Regional Agency for Science and Technology (Comunidad Autónoma de la Región de Murcia, CARM), through the grant number 20855/PI/18); and, by the Institute of Health “Carlos III” (ISCIII), and co-funded by the Fondo Europeo de Desarrollo Regional-FEDER (grant number PI20/00505). M.A.M.-S. was supported by a PFIS contract from the ISCIII (FI21/00003, ISCIII, Spain; co-funded by the Fondo Europeo de Desarrollo Regional-FEDER). B.R.-M. was supported by the “Miguel Servet Type I” program (CP19/00098, ISCIII, Spain; co-funded by the Fondo Europeo de Desarrollo Regional-FEDER).
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/21/13400
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPhytochemicals
dc.subjectLeukemia
dc.subjectCancer
dc.subjectSulforaphane
dc.subjectPhenolic compounds
dc.subjectBrassica
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleAnti-leukemic activity of Brassica-derived bioactive compounds in HL-60 myeloid leukemia cells
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublicationc5d54ef3-bd3b-4330-b4a1-252ab278925b
relation.isAuthorOfPublication.latestForDiscoveryc5d54ef3-bd3b-4330-b4a1-252ab278925b
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