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Differential tissue expression of enhanced green fluorescent protein in ‘Green mice’

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dc.contributor.authorMa, De-Fu
dc.contributor.authorTezuka, Hideo
dc.contributor.authorKondo, Tetsuo
dc.contributor.authorSudo, Katsuko
dc.contributor.authorNiu, Dong-Feng
dc.contributor.authorNakazawa, Tadao
dc.contributor.authorKawasaki, Tomonori
dc.contributor.authorYamane, Tetsu
dc.contributor.authorNakamura, Nobuki
dc.contributor.authorKatoh, Ryohei
dc.date.accessioned2015-10-06T14:37:14Z
dc.date.available2015-10-06T14:37:14Z
dc.date.issued2010
dc.description.abstractIn order to clarify tissue expression of enhanced green fluorescent protein (EGFP) in ‘green mice’ from a transgenic line having an EGFP cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer, we studied the expression of EGFP in various organs and tissues from these ‘green mice’ by immunohistochemistry with anti- EGFP antibody in conjunction with direct observation for EGFP fluorescence using confocal laser scanning microscopy. On immunohistochemical examination and on direct observation by confocal laser scanning microscopy, the level of EGFP expression varied among organs and tissues. EGFP expression was diffusely and strongly observed in the skin, pituitary, thyroid gland, parathyroid gland, heart, gall bladder, pancreas, adrenals and urinary bladder. There was only sporadic and weak expression of EGFP in the epithelium of the trachea, bronchus of the lung, stratified squamous epithelium and gastric glands of the stomach, hepatic bile ducts of the liver, glomeruli and renal tubules of the kidney and endometrial glands of the uterus. Furthermore, EGFP was only demonstrated within the goblet and paneth cells in the colon and small intestine, the tall columnar cells in the ductus epididymis, and the leydig cells in the testis. In conclusion, our results show that EGFP is differentially expressed in organs and tissues of ‘green mice’, which indicates that ‘green mice’ may prove useful for research involving transplantation and tissue clonality.es
dc.formatapplication/pdfes
dc.format.extent6es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/46509
dc.languageenges
dc.publisherMurcia: F. Hernándezes
dc.relation.ispartofHistology and histopathology, Vol. 25, nº 6 (2010)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectImmunohistochemistryes
dc.subject.other616 - Patología. Medicina clínica. Oncologíaes
dc.titleDifferential tissue expression of enhanced green fluorescent protein in ‘Green mice’es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.25, nº6 (2010)