A differential structure approach to membrane segmentation in electron tomography

Martinez-Sanchez, Antonio; Inmaculada, García; Fernández, Jose-Jesús

Publication:
A differential structure approach to membrane segmentation in electron tomography

dc.contributor.author	Martinez-Sanchez, Antonio
dc.contributor.author	Inmaculada, García
dc.contributor.author	Fernández, Jose-Jesús
dc.contributor.department	Ingeniería de la Información y las Comunicaciones
dc.date.accessioned	2023-11-30T07:56:53Z
dc.date.available	2023-11-30T07:56:53Z
dc.date.issued	2011-09
dc.description	© 2011. Elsevier. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Journal of Structural Biology. To access the final edited and published work see https://doi.org/10.1016/j.jsb.2011.05.010	es
dc.description.abstract	Electron tomography allows three-dimensional visualization of cellular landscapes in molecular detail. Segmentation is a paramount stage for the interpretation of the reconstructed tomograms. Although several computational approaches have been proposed, none has prevailed as a generic method and thus segmentation through manual annotation is still a common choice. In this work we introduce a segmentation method targeted at membranes, which define the natural limits of compartments within biological specimens. Our method is based on local differential structure and on a Gaussian-like membrane model. First, it isolates information through scale-space and finds potential membrane-like points at a local scale. Then, the structural information is integrated at a global scale to yield the definite segmentation. We show and validate the performance of the algorithm on a number of tomograms under different experimental conditions.	es
dc.format	application/pdf	es
dc.format.extent	49	es
dc.identifier.citation	Journal of Structural Biology Vol. 175, Issue 3, September 2011, Pages 372-383
dc.identifier.doi	https://doi.org/10.1016/j.jsb.2011.05.010
dc.identifier.issn	1047-8477
dc.identifier.uri	http://hdl.handle.net/10201/136263
dc.language	eng	es
dc.relation	This work has been partially supported by the Spanish Ministry of Science (MCI-TIN2008-01117), the Spanish National Research Council (CSIC-PIE200920I075) and J. Andalucia (P10-TIC-6002). A.M.S. is a fellow of the Spanish FPI programme.	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.subject	Segmentation	es
dc.subject	Image Processing	es
dc.subject	Electron Tomography	es
dc.subject	Membrane	es
dc.subject	ScaleSpace	es
dc.title	A differential structure approach to membrane segmentation in electron tomography	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es