Publication: Ethylnitrosourea ENU - induced apoptosis in the rat fetal tissues
Loading...
Date
2000
Authors
Katayama, K. ; Ishigami, M. ; Uetsuka, K. ; Nakayama, Hiroyuki ; Doi, K.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Ethylnitrosourea (ENU), a well known DNA
alkylating agent, induces anomalies in the central
nervous system (CNS), craniofacial tissues and male
reproductive organs. In this study, pregnant rats were
treated with 60 mg/kg ENU at day 13 of gestation, and
their fetuses were examined from 1 to 48 hours after
treatment (HAT) to find a clue for clarifying the
mechanisms of the ENU fetotoxicity and teratogenicity.
From 3 to 12 HAT, the moderate to marked increase in
the number of pyknotic cells was detected in the fetal
CNS, craniofacial mesenchymal tissues, gonads and so
on. These pyknotic cells had nuclei positively stained by
the TUNEL method, which is widely used for the
detection of apoptotic nuclei, and they also showed
electron microscopic characteristics identical to those of
apoptotic cells. The present results strongly suggest that
excess cell death by apoptosis in the fetal CNS,
craniofacial tissues and gonads may have a close relation
to the later occurrence of anomalies reported in these
tissues following ENU-administration.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.