Mycoplasma bovis in Spanish Cattle Herds: Two Groups of Multiresistant Isolates Predominate, with One Remaining Susceptible to Fluoroquinolones

Nouvel, Laurent-Xavier; Baranowski, Eric; Gómez-Martín, Ángel; Sánchez López, Antonio; Citti, Christine; de la Fe Rodríguez, Christian; García-Galán Pérez, Ana

Publication:
Mycoplasma bovis in Spanish Cattle Herds: Two Groups of Multiresistant Isolates Predominate, with One Remaining Susceptible to Fluoroquinolones

dc.contributor.author	Nouvel, Laurent-Xavier
dc.contributor.author	Baranowski, Eric
dc.contributor.author	Gómez-Martín, Ángel
dc.contributor.author	Sánchez López, Antonio
dc.contributor.author	Citti, Christine
dc.contributor.author	de la Fe Rodríguez, Christian
dc.contributor.author	García-Galán Pérez, Ana
dc.contributor.department	Sanidad Animal
dc.date.accessioned	2023-11-24T11:37:56Z
dc.date.available	2023-11-24T11:37:56Z
dc.date.issued	2020-07-07
dc.description	© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).This document is the Accepted version of a Published Work that appeared in final form in Pathogens. To access the final edited and published work see https://doi.org/10.3390/pathogens9070545	es
dc.description.abstract	Mycoplasma bovis is an important bovine pathogen causing pneumonia, mastitis, and arthritis and is responsible for major economic losses worldwide. In the absence of an efficient vaccine, control of M. bovis infections mainly relies on antimicrobial treatments, but resistance is reported in an increasing number of countries. To address the situation in Spain, M. bovis was searched in 436 samples collected from beef and dairy cattle (2016–2019) and 28% were positive. Single-locus typing using polC sequences further revealed that two subtypes ST2 and ST3, circulate in Spain both in beef and dairy cattle, regardless of the regions or the clinical signs. Monitoring of ST2 and ST3 isolates minimum inhibitory concentration (MIC) to a panel of antimicrobials revealed one major difference when using fluoroquinolones (FQL): ST2 is more susceptible than ST3. Accordingly, whole-genome sequencing (WGS) further identified mutations in the gyrA and parC regions, encoding quinolone resistance-determining regions (QRDR) only in ST3 isolates. This situation shows the capacity of ST3 to accumulate mutations in QRDR and might reflect the selective pressure imposed by the extensive use of these antimicrobials. MIC values and detection of mutations by WGS also showed that most Spanish isolates are resistant to macrolides, lincosamides, and tetracyclines. Valnemulin was the only one effective, at least in vitro, against both STs.	es
dc.format	application/pdf	es
dc.format.extent	19	es
dc.identifier.doi	https://doi.org/10.3390/pathogens9070545
dc.identifier.eisbn	Pathogens 2020, 9(7), 545	es
dc.identifier.issn	2076-0817
dc.identifier.uri	http://hdl.handle.net/10201/136026
dc.language	eng	es
dc.publisher	MDPI	es
dc.relation	This research has been supported by the Spanish Ministry of Economy and Competitiveness (Spanish Government) co-financed by FEDER funds, project AGL2016-76568-R.	es
dc.relation.publisherversion	https://www.mdpi.com/2076-0817/9/7/545	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Mycoplasma bovis	es
dc.subject	Minimum inhibitory concentration	es
dc.subject	Antimicrobial resistance	es
dc.subject	Mutations	es
dc.subject	Spain	es
dc.title	Mycoplasma bovis in Spanish Cattle Herds: Two Groups of Multiresistant Isolates Predominate, with One Remaining Susceptible to Fluoroquinolones	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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