Publication: Expression of acyl-CoA synthetase 5 in human epidermis
Authors
Gaisa, N.T. ; Köster, J. ; Reinartz, A. ; Ertmer, K. ; Ehling, J. ; Raupach, K. ; Perez-Bouza, A. ; Knüchel, R. ; Gassler, N.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The human epidermis is characterized by a
constant renewal of keratinocytes embedded in a matrix
enriched with lipids. Numerous proteins involved in
lipid metabolism are found in human epidermis,
especially in keratinocytes. Long-chain acyl-CoA
derivatives, which are catalyzed by human ACSL5, are
important metabolites in several biochemical pathways,
including ceramide de novo synthesis. The aim of the
present study was to investigate expression of acyl-CoA
synthetase isoform 5 (ACSL5) in human epidermis by an
in situ, as well as a molecular approach. We show that
ACSL5 mRNA and protein are found in human
epidermis, as well as in non-differentiated and
differentiated HaCaT cells. Keratinocytes of stratum
spinosum are the main source for ACSL5 expression in
both meshed facial or abdominal skin and ridged skin of
upper or lower extremities including TUNEL-positive
cells in upper cellular layers. Single keratinocytes of
chronic solar-exposed meshed facial epidermis
occasionally display a stronger ACSL5 immunostaining.
In conclusion, our study indicates that epidermal ACSL5
expression might be involved in differentiation and the
stress response of keratinocytes.
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