Publication: Time-course expression of metallothioneins
and tissue metals in chronic relapsing form of
experimental autoimmune encephalomyelitis
Authors
Jakovac, Hrvoje ; Grebic, Damir ; Tota, Marin ; Barac-Latas, Vesna ; Mrakovčić-Šutić, Ines ; Milin, Čedomila ; Radosevic-Stasic, Biserka
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
To elucidate the role of metallothioneins
(MTs) in the pathomechanisms of autoimmune CNS
disorders we estimated the expression of MTs I+II and
the tissue concentrations of Zn2+ and Cu2+ in the brain,
spinal cord (SC) and in the liver during the periods of
attacks and remissions in chronic relapsing experimental
autoimmune encephalomyelitis (CR-EAE). Disease was
induced in the genetically susceptible Dark Agouti (DA)
rats by subcutaneous injection of bovine brain
homogenate in CFA. Control rats were treated with CFA.
The data, obtained by clinical assessment,
immunohistochemistry and inductivity coupled plasma
spectrometry, have shown that during the first attack (on
the 12th day) MTs I+II were markedly upregulated in
subarachnoid regions and perivascular space on
astrocytes, microglia and on spinal neurons.
Simultaneously, the concentrations of zinc in the SC and
zinc and copper in the liver have found to be increased.
During the second attack (on the 22nd day) a new
overexpression of MTs was found in the cerebellum, in
sulcus hippocampi, in spinal neurons and particularly in
hepatocytes around the central vein. Concomitantly, in
the brain and SC the concentration of copper increased.
The data point to a neuroprotective role of MTs and
to an important regulatory role of essential metals and
hepatic MTs in the pathogenesis of CR-EAE
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