Publication:
Cytochemical and biochemical evidences for a complex tridimensional structure of the hamster zona pellucida

Simple item page
dc.contributor.authorSaavedra Leos, María Dolores
dc.contributor.authorGutiérrez Gallego, Ricardo
dc.contributor.authorFayrer Hosken, Richard
dc.contributor.authorBallesta Germán, José
dc.contributor.authorAvilés Sánchez, Manuel
dc.contributor.authorCastells Mora, María Teresa
dc.contributor.authorIzquierdo Rico, María José
dc.contributor.authorJíménez Movilla, María
dc.contributor.authorMartínez Alonso, Emma
dc.contributor.otherBiología Celular
dc.date.accessioned2013-09-24T11:35:08Z
dc.date.available2013-09-24T11:35:08Z
dc.date.issued2009
dc.description.abstractZona pellucida (ZP) is an extracellular matrix that surrounds eggs and pre-implantation embryos and is required for in vivo fertility. A key event in successful fertilization is sperm binding to the surface of the ZP. It has been previously described that the hamster sperm binds mainly the outer region of the ZP which corresponds to the porous region in contact with the cumulus cells. Using ultrastructural cytochemistry approaches with an antibody developed against porcine ZP, this study shows that the pig ZP shares epitopes with some rodent species like hamster, rat and mouse. In the hamster, these epitopes are located mainly in the outer region of the ZP of preovulatory and ovulated oocytes. By means of biochemical approaches it was demonstrated that 1) the antibody is specific for the native hamster ZP3, 2) four different bands with a molecular weight of 67, 60, 48 and 38 kDa after Nlinked deglycosylation suggesting that the hamster ZP is formed by four proteins, and 3) the different composition observed in the outer region of the hamster ZP could be due to a specific supramolecular structure that makes some epitopes accessible for the antibodies. In summary, this study provides evidence that the different composition observed in the different regions of the ZP is mediated by a different organization of the components of the ZP produced during the oocyte maturation. This different organization could be responsible for the different sperm binding affinity observed for sperm to the outer region versus the inner region of the ZP.es
dc.formatapplication/pdfes
dc.format.extent11es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/36021
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectCytochemistryes
dc.subjectHamsteres
dc.subject.other576 - Biología celular y subcelular. Citologíaes
dc.titleCytochemical and biochemical evidences for a complex tridimensional structure of the hamster zona pellucidaes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicationeb2d72d3-a1d5-48cc-b71f-4aaf60a9aeac
relation.isAuthorOfPublicationd6ad2235-d5ca-4574-85eb-964da3c5c44c
relation.isAuthorOfPublicationb0205f82-a44c-47b6-8061-b55efb4e6717
relation.isAuthorOfPublication0c5bf0d3-e651-4120-8e9e-18633c9be135
relation.isAuthorOfPublication3fd5eb36-2d6e-4f9e-8e3f-fa44f8982697
relation.isAuthorOfPublication.latestForDiscoveryeb2d72d3-a1d5-48cc-b71f-4aaf60a9aeac
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Cytochemical and biochemical evidences for a complex tridimensional structure of the hamster zona pellucida.pdf
Size:
1.74 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Histology and histopathology Vol.24, nº5 (2009)