Publication: Puerarin and zinc additively prevent mandibular bone loss through inhibiting osteoclastogenesis in ovariectomized rats
Authors
Liu, Hao ; Li, Wei ; Jia, Shengnan ; Li, Binbin
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-855
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info:eu-repo/semantics/article
Description
Abstract
Puerarin and zinc play a key role in
preventing osteoporotic-related bone loss. Previous
research on puerarin or zinc mainly focused on the antiosteoporotic effects of long bone. However, it is obscure
for puerarin or zinc to prevent mandibular osteoporosis.
Here, we explore the effects on additive coadministration of puerarin and zinc on preventing mandibular
bone loss in ovariectomized rats, and evaluate the
underlying mechanisms ex vivo. Rats were ovariectomized and administrated puerarin, zinc or both. After 12
weeks, bone mineral density (BMD) and histomorphometry of mandibles were measured by micro-CT. The
mechanical properties were determined using a threepoint bending test. Then, osteogenic differentiation of
primary bone marrow stromal cells (BMSCs) and
osteoclastogenesis of bone marrow mononuclear were
performed ex vivo. The culture supernatant and serum
level of bone biochemical markers including
osteoprotegerin (OPG), osteopontin (OPN), receptor
activator of nuclear factor (NF)-κB ligand (RANKL),
and tartrate-resistant acid phosphatase (TRAP) were
detected by ELISA. Culture supernatant and serum
levels of calcium were measured using a Plasma
Emission Spectrometer. One-way ANOVA was used for
statistical analyses. The results showed that
administration of puerarin plus zinc prevented the
decrease in mandibular BMD and bone morphometrical
parameters more effectively than single use of puerarin
or zinc (p<0.05), which was similar to the biomechanical
tests (p<0.05). Furthermore, puerarin and zinc additively
up-regulated OPG, OPN protein levels, Ca ion level and
down-regulated RANKL, TRAP protein levels. In
conclusion, puerarin and zinc additively prevent
mandibular bone loss through inhibiting osteoclastogenesis in ovariectomized rats, which will shed more
light on the potential use of puerarin and zinc in the
prevention/treatment of oral bone loss clinically.
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Citation
Histology and Histopathology, Vol.32, nº8, (2017)
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