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Cellular compartmentation of lysozyme and a-amylase in the mouse salivary glands. lmmunogold approaches at light and electron microscopy level

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dc.contributor.authorMarchetti, L.es
dc.contributor.authorGabrielli, M.G.es
dc.contributor.authorMaterazzi, G.
dc.contributor.authorMenghi, Giovanna
dc.date.accessioned2011-02-22T11:21:26Z
dc.date.available2011-02-22T11:21:26Z
dc.date.issued2000
dc.description.abstractThe research was planned to study the subcellular distribution of enzymatic secretory products within the secretory structures of the mouse major salivary glands at light and electron microscopy level by immunogold silver stain (IGSS) technique and doublesided post-embedding immunogold binding and silver amplification in order to speculate about their compartmentation. In particular, we experimented the above immunogold labeling approaches to localize the lysozyme and to verify its distribution patterns in relation to another secretion enzyme, a-amylase. Co-presence of lysozyme and a-amylase was observed in the convoluted granular tubule cells of the submandibular gland and in the demilunar cells of the sublingual gland as well as in the electron-dense regions of the mottled secretory granules in the parotid gland. Exclusive binding patterns of lysozyme were observed in the acinar cells of the submandibular and sublingual glands where a-amylase did not occur.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/19316
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectLysozymees
dc.subjectMousees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleCellular compartmentation of lysozyme and a-amylase in the mouse salivary glands. lmmunogold approaches at light and electron microscopy leveles
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.15, nº 2 (2000)