Publication: Detection of glycoconjugates in the ductus epididymis of the prepubertal and adult horse by lectin histochemistry
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Date
1997
Authors
Parillo, F. ; Stradaioli, G. ; Verini Supplizi, A. ; Monaci, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This paper describes an approach for
studying the structure of glycoconjugates found in the
principal cells lining the epididymal duct in adult and
prepubertal horses, using ten different lectin horseradish
coijugates: Con-A, L C ~W, GA, GSA-11, SBA, PNA,
RCA-1, DBA, UEA-1, and LTA. Saponification and
sialidase procedures, followed by lectin binding, were
employed to visualize the distribution and to reveal the
sequence of sialoglycoconjugates in ductus epididymis.
In the adult horse the results demonstrated variations in
the content and distribution of glycosidic residues of
glycoconjugates in different epididymal regions (caput,
corpus, cauda) and vas deferens, suggesting that each
epididymal segment has a specific function. In
particular, staining of the Golgi-zone in the principal
cells lining corpus epididymis was interpreted as
evidence for synthesis and secretion of glycoconjugates
and sialoglycoconjugates. In the prepubertal horse, only
the glycocalyx of the epithelial cells lining the
epididymal duct showed reactivity toward the different
lectins used, suggesting hormonal regulation of the
epididymis activity. Additionally, the heterogeneity of
the lectin staining pattern of the adult horse epididymis
reported in this investigation also suggests the existence
of different functional segments along the epididymal
duct.
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