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The tick-derived rBmTI-A protease inhibitor attenuates the histological and functional changes induced by cigarette smoke exposure

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dc.contributor.authorLourenço, Juliana D.
dc.contributor.authorIto, Juliana T.
dc.contributor.authorCervilha, Daniela A.B.
dc.contributor.authorSales, Davi S.
dc.contributor.authorRiani, Alyne
dc.contributor.authorSuehiro, Camila L.
dc.contributor.authorGenaro, Isabella S.
dc.contributor.authorDuran, Adriana
dc.contributor.authorPuzer, Luciano
dc.contributor.authorMartins, Milton A.
dc.contributor.authorSasaki, Sérgio D.
dc.contributor.authorLopes, Fernanda D.T.Q.S.
dc.date.accessioned2022-04-05T10:18:17Z
dc.date.available2022-04-05T10:18:17Z
dc.date.issued2018
dc.description.abstractIntroduction. Smoking is the main risk factor for chronic obstructive pulmonary disease development and cigarette smoke (CS) exposure is considered an important approach to reproduce in rodents this human disease. We have previously shown that in an elastase-induced model of emphysema, the administration of a protease inhibitor (rBmTI-A) prevented and attenuated tissue destruction in mice. Thus, in this study we aimed to verify the effects of rBmTI-A administration on the physiopathological mechanisms of CS-induced emphysema. Methods. Mice (C57BL/6) were exposed to CS or room air for 12 weeks. In this period, 3 nasal instillations of rBmTI-A inhibitor or its vehicle were performed. After euthanasia, respiratory mechanics were evaluated and lungs removed for analysis of mean linear intercept, volume proportion of collagen and elastic fibers, density of polymorphonuclear cells, macrophages, and density of positive cells for MMP-12, MMP-9, TIMP-1 and gp91phox. Results. The rBmTI-A administration improved tissue elastance, decreased alveolar enlargement and collagen fibers accumulation to control levels and attenuated elastic fibers accumulation in animals exposed to CS. There was an increase of MMP12, MMP-9 and macrophages in CS groups and the rBmTIA only decreased the number of MMP-12 positive cells. Also, we demonstrated an increase in gp91phox in CS treated group and in TIMP-1 levels in both rBmTI-A treated groups. Conclusion. In summary, the rBmTI-A administration attenuated emphysema development by an increase of gp91phox and TIMP-1, accompanied by a decrease in MMP-12 levels.es
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dc.identifier.citationHistology and Histopathology, Vol.33, nº3, (2018)
dc.identifier.doiDOI: 10.14670/HH-11-927
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/118731
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectProtease inhibitores
dc.subjectCigarette smokees
dc.subjectEmphysemaes
dc.subjectMetalloproteaseses
dc.subjectAnimal modelses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleThe tick-derived rBmTI-A protease inhibitor attenuates the histological and functional changes induced by cigarette smoke exposurees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.33, nº3 (2018)