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Fluoxetine may worsen hyperoxia-induced lung damage in neonatal rats

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dc.contributor.authorPorzionato, Andrea
dc.contributor.authorZaramella, Patrizia
dc.contributor.authorMacchi, Verónica
dc.contributor.authorGrisafi, Davide
dc.contributor.authorSalmaso, Roberto
dc.contributor.authorBaraldi, Maura
dc.contributor.authorFornaro, Enrica
dc.contributor.authorTassone, Evelyn
dc.contributor.authorMasola, Valentina
dc.contributor.authorOnisto, Maurizio
dc.contributor.authorChiandetti, Lino
dc.contributor.authorDe Caro, Raffaele
dc.date.accessioned2017-11-09T15:56:48Z
dc.date.available2017-11-09T15:56:48Z
dc.date.issued2012
dc.description.abstractFluoxetine shows controversial lung effects as it prevents pulmonary hypertension in adult rats but exposure during gestation causes pulmonary hypertension in neonatal rats. In the present study, we tested the null hypothesis that the antidepressant drug fluoxetine does not modify the development of bronchopulmonary dysplasia (BPD) in neonatal rats. Experimental categories included I: room air (controls) with daily injection of saline; II: room air with daily injection of 10 mg/kg fluoxetine, i.p., during two weeks; III: 60% oxygen with daily injection of saline; and IV: 60% oxygen with daily injection of 10 mg/kg fluoxetine, i.p., during two weeks. Hyperoxia resulted in significant reduction in alveolar density and an increase in pulmonary endocrine cells, as well as increases in muscle layer areas of bronchi and arteries. Fluoxetine treatment generated a further increase in muscularisation and did not significantly modify the hyperoxia-induced reductions in alveolar density and increases in the endocrine cells. In hyperoxia, Real-Time PCR showed a lower pulmonary expression of vascular endothelial growth factor (VEGF) with no significant changes in the expression of matrix metalloproteinases (MMP) 2 and 12. Fluoxetine did not affect VEGF or MMP-2 expression but it significantly increased MMP-12 mRNA in both normoxic and hyperoxic groups. Zymographic analysis of MMP-2 activity in bronchoalveolar fluid showed a significantly reduced MMP-2 activity in hyperoxia, while fluoxetine treatment restored MMP-2 activity to levels comparable with the normoxic group. In conclusion, our data show that fluoxetine may worsen bronchial and arterial muscularisation during development of BPD and may up-regulate MMP expression or activity.es
dc.formatapplication/pdfes
dc.format.extent12es
dc.identifier.citationHistology and histopathology, Vol. 27, n.º 12 (2012)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/54473
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectMatrix metalloproteinasesen_EN
dc.subjectBroncho-pulmonar dysplasiaen_EN
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleFluoxetine may worsen hyperoxia-induced lung damage in neonatal ratses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.27, nº12 (2012)