Publication: Effect of short-term betamethasone administration on the regeneration process of tissue-engineered bone
Authors
Chihara, Takahiro ; Zhang, Yiming ; Li, Xianqi ; Shinohara, Atsushi ; Kagami, Hideaki
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-193
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info:eu-repo/semantics/article
Description
Abstract
Local inflammation at the transplanted site of
tissue-engineered bone may cause apoptosis of the
transplanted cells, thus negatively affecting bone
regeneration. To maximize the efficacy of bone tissue
engineering, the local effect of short-term corticosteroid
administration at the transplanted site of tissue-
engineered bone was studied with respect to the
expression of inflammatory cytokines. Compact bone-
derived cells from mouse leg bones were isolated,
cultured and seeded onto β-tricalcium phosphate
granules. The constructs were transplanted to the back of
syngeneic mice. Betamethasone sodium phosphate was
administered intraperitoneally to an experimental
(betamethasone) group, whereas the same amount of
saline was administered to a control group. When
betamethasone was administered three times
(immediately after operation and 12 hours and 24 hours
after transplantation), the number of SP7/osterix-positive
osteoblasts was larger in the betamethasone group. Three
times of betamethasone administration (immediately
after operation and 12 hours and 24 hours after
transplantation) did not change the number of apoptotic
cells and osteoclasts, but showed a slight upregulation of
IL-4 and a downregulation of IL-6. However, 7 doses of
betamethasone administration (over 7 consecutive days)
increased the number of apoptotic cells and osteoclasts,
which was correlated with a downregulation of IL-4 and
an upregulation of IL-6. TNF-α expression levels
showed no significant differences between the two
groups. The results showed beneficial effects of 3
betamethasone administrations for bone regeneration
therapy but contrary effects when betamethasone was
administered 7 times due to the downregulation of anti-
inflammatory cytokines (IL-4) and the upregulation of
inflammatory cytokines (IL-6). As a conclusion, our
results suggested the importance of the cautious usage of
corticosteroids to control local inflammation at
transplanted sites in bone tissue engineering
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Citation
Histology and Histopathology Vol. 35, nº7 (2020)
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