Publication:
Effect of Superovulation Treatment on Oocyte's DNA Methylation

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dc.contributor.authorS. Lopes, Jordana
dc.contributor.authorIvanova, Elena
dc.contributor.authorRuiz, Salvador
dc.contributor.authorAndrews, Simon
dc.contributor.authorKelsey, Gavin
dc.contributor.authorCoy, Pilar
dc.contributor.departmentDidáctica y Organización Escolares
dc.date.accessioned2025-09-05T10:42:52Z
dc.date.available2025-09-05T10:42:52Z
dc.date.issued2022-12-18
dc.description© 2022 The Author(s)____ This document is the published version of a published work that appeared in final form in International Journal of Molecular Sciences____ This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0 ____ To access the final edited and published work see: https://doi.org/10.3390/ijms232416158es
dc.description.abstractControlled ovarian stimulation is a necessary step in some assisted reproductive procedures allowing a higher collection of female gametes. However, consequences of this stimulation for the gamete or the offspring have been shown in several mammals. Most studies used comparisons between oocytes from different donors, which may contribute to different responses. In this work, we use the bovine model in which each animal serves as its own control. DNA methylation profiles were obtained by single-cell whole-genome bisulfite sequencing of oocytes from pre-ovulatory unstimulated follicles compared to oocytes from stimulated follicles. Results show that the global percentage of methylation was similar between groups, but the percentage of methylation was lower for non-stimulated oocytes in the imprinted genes APEG3, MEG3, and MEG9 and higher in TSSC4 when compared to stimulated oocytes. Differences were also found in CGI of imprinted genes: higher methylation was found among non-stimulated oocytes in MEST (PEG1), IGF2R, GNAS (SCG6), KvDMR1 ICR UMD, and IGF2. In another region around IGF2, the methylation percentage was lower for non-stimulated oocytes when compared to stimulated oocytes. Data drawn from this study might help to understand the molecular reasons for the appearance of certain syndromes in assisted reproductive technologies-derived offspring.es
dc.formatapplication/pdfes
dc.format.extent19es
dc.identifier.citationInternational Journal of Molecular Sciences 2022, 23, 16158
dc.identifier.doihttps://doi.org/10.3390/ijms232416158
dc.identifier.issnPrint.: 1661-6596
dc.identifier.issnElectronic.: 1422-0067
dc.identifier.urihttp://hdl.handle.net/10201/158172
dc.languageenges
dc.publisherMDPIes
dc.relationThis research was funded by European Union Horizon 2020 Marie Skłodowska-Curie Action (Rep-Biotech 675526); Spanish Ministry of Economy and Competitiveness (MINECO) & European Regional Development Fund (FEDER) (AGL2015-66341-R); Spanish Ministry of Science and Innovation (PID2020-113366RB-I00); Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia (20040/GERM/16); and the UK Biotechnology and Biological Sciences Research Council (BBS/E/B/000C0423) and Medical Research Council (MR/K011332/1)es
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/24/16158es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectOocytees
dc.subjectDNA methylationes
dc.subjectOvarian stimulationes
dc.subjectARTes
dc.subjectEpigeneticses
dc.titleEffect of Superovulation Treatment on Oocyte's DNA Methylationes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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