Publication: Immunohistochemical localization of renin, NO synthase-1, and cyclooxygenase-2 in rodent kidney
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Date
2008
Authors
Ichii, Osamu ; Yabuki, Akira ; Ojima, Toshimichi
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The renin-angiotensin system (RAS) and
tubuloglomerular feedback (TGF) are central to the
maintenance of blood pressure and body fluid
composition. Renin, NO synthase-1 (NOS-1), and
cyclooxygenase-2 (COX-2) are key regulators of the
RAS and TGF. In the present study, to investigate
species-specific differences in the RAS and TGF, we
immunohistochemically and morphometrically
investigated the localization of renin, NOS-1, and COX-
2 in the kidneys of various laboratory rodents and
comparing males with females (DBA/2Cr mice, F344/N
rats, Syrian hamsters, MON/JmsGbs gerbils and Hartley
guinea pigs). In all animals, renin-positive
immunoreactions were observed in the vascular walls of
afferent arterioles. Renin immunoreactions appeared to
be more widely distributed in mice. Mice had a greater
number of renin-positive arterioles than other species.
NOS-1-positive reactions were detected in the macula
densa (MD) of all animals. Mice had the greatest number
of NOS-1-positive MD cells. In addition to NOS-1-
positive reactions, COX-2-positive reactions were
observed in the MD of mice, rats, hamsters and gerbils.
Interestingly, guinea pigs had no COX-2-positive MD
cells. Rats had the greatest number of COX-2-positive MD cells. In nephron segments excluding the MD, the
immunohistochemical localization of NOS-1 and COX-2
differed markedly among not only species but also sexes
within the same species. In conclusion, we determined
that localization of renin, NOS-1, and COX-2 showed
large species- and sex-related differences. These data
suggest that the regulation mechanisms of the RAS and
TGF via renin, NOS-1, and COX-2 differ among
rodents.
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