Publication: Effect of regenerative factor rich plasma, P substance and fetal calf serum on the growth of epithelial cells in the cornea. Comparative experimental study
Authors
Márquez de Aracena del Cid, R. ; Pérez Ordoñez, A.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Purpose. The goal of this study was to
evaluate the experimental effectiveness of Regenerative
Factor Rich Plasma (RFRP) of human blood versus Fetal
Bovim Serum (FBS) and neuropeptide Substance P (SP)
on corneal epithelium cell proliferation. Method. Rabbit
corneal epithelium cell (CCL-60) growth was compared
between different RFRP fractions, FBS and with the
neuropeptide Substance P. The ability of the RFRP
fractions and SP to revert the inhibitory effect of the CsA
was also evaluated. Results. All groups showed an
increase (p<0.001) in corneal epithelial cell growth
compared with the control group. The maximum
capacity of cell growth was obtained with dilutions of
50% in the FBS, RFRP-I, RFRP -II, RFRP-III groups
and with 100nM of SP. The highest growth was observed
with 50% FBS, RFRP-I and RFRP-II. The group with
SP and RFRP-III had significantly lower growth
(p<0.001). When the NK1 receptor antagonist CsA was
added at a dose of IC50, we found a significant decrease
in cell growth (p<0.001) in all culture conditions,
including the control group. The decrease was similar in
all groups, but was especially pronounced in RFRP-II.
RFRP I, II and III promoted growth more than SFB
10%. Conclusion. The RFRP of human blood promotes
the growth of corneal epithelial cells in a significantly
more efficient manner than FSB and SP. RFRP can be
effective both in cell cultures and stem cell cultures.
Citation
Histology and Histopathology, vol. 28, nº 8 (2013)
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