Publication:
Differential expression of proteins related to smooth muscle cells and myofibroblasts in human thoracic aortic aneurysm

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dc.contributor.authorForte, Amalia
dc.contributor.authorDella Corte, Alessandro
dc.contributor.authorGrossi, Mario
dc.contributor.authorBancone, Ciro
dc.contributor.authorMaiello, Ciro
dc.contributor.authorGalderisi, Umberto
dc.contributor.authorCipollaro, Marilena
dc.date.accessioned2018-05-17T10:45:29Z
dc.date.available2018-05-17T10:45:29Z
dc.date.issued2013
dc.description.abstractObjectives: Increasing knowledge is required for a better comprehension of the etiology of thoracic aortic aneurysm (TAA). The aim of this study was to highlight the modulations in vascular cell phenotypes, including myofibroblasts (MFs), in human TAA specimens compared to healthy aortas. Methods: histology, RT-PCR and immunohistochemical analysis of a panel of molecules, including EDA Fibronectin (Fn), smoothelin, CD34 and alpha-smooth muscle actin (alpha-SMA), selected on the basis of their informative potential as markers of smooth muscle cells (SMCs) and MF phenotypic modulation, were performed on all samples. Results: The media of TAAs was characterized by the absence of smoothelin, the unaltered expression of alpha-SMA accompanied by an alteration of its distribution pattern, and by the activated expression of the ED-A isoform of Fn. We found a concentration of round-shaped cells exclusively in the adventitia and in the perivascular tissue of TAAs, also rich in vasa vasorum, largely expressing alpha-SMA, while a sub-population also expressed ED-A Fn and CD34. CD34 was expressed by several cells in the intima of TAAs, together with cells expressing cytoplasmatic EDA Fn and alpha-SMA in comparison to healthy aortas. Conclusion: TAA specimens show an altered expression and localization of SMC and MF differentiation markers in comparison to healthy aortas, with possible implications on remodeling.es
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dc.format.extent9es
dc.identifier.citationHistology and Histopathology, vol. 28, nº 6. (2013)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/58486
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectThoracic aortic aneurysmes
dc.subjectMyofibroblastses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleDifferential expression of proteins related to smooth muscle cells and myofibroblasts in human thoracic aortic aneurysmes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.28, nº 6 (2013)