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Bioaccumulation of mycotoxins in human forensic liver and animal liver samples using a green sample treatment

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dc.contributor.authorCastell Martínez, Ana
dc.contributor.authorArroyo Manzanares, Natalia
dc.contributor.authorCampillo Seva, Natalia
dc.contributor.authorTorres Sánchez, Carmen
dc.contributor.authorFenoll, José
dc.contributor.authorViñas López-Pelegrin, Pilar
dc.contributor.departmentQuímica Analítica
dc.date.accessioned2025-10-23T07:15:15Z
dc.date.available2025-10-23T07:15:15Z
dc.date.copyright© 2022 The Author(s)
dc.date.issued2023-11-23
dc.description.abstractThe investigation of the mycotoxin bioaccumulation in human and animals is of wide relevance due to the potential toxicity associated with these secondary metabolites. This study proposes an analytical methodology consisting of salting-out liquid–liquid extraction (SALLE) followed by dispersive liquid–liquid microextraction (DLLME) for the determination of 13 mycotoxins: aflatoxins (G1, G2, B1 and B2), enniatins (A, A1, B and B1), beauvericin, HT-2 and T-2 toxins, zearalenone and deoxynivalenol, in human and animal liver. A targeted analysis was performed by high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS), as well as the screening of derived metabolites by ultrahigh performance LC and high-resolution mass spectrometry (UHPLC-HRMS). The proposed method was in-home validated, and trueness was verified by apparent recovery studies with values between 94 and 110 %. Furthermore, suitable linearities were obtained by the proposed method for all the mycotoxins and detection and quantification limits allow the mycotoxin monitoring at the low levels expected in biological samples. Repeatability and intermediate precision were calculated at two concentration levels, obtaining values of relative standard deviation below 9.5 %. The proposed methodology allowed to study the bioaccumulation of mycotoxins in both human and animal liver, demonstrating the presence of emergent mycotoxins in all liver samples analyzed, specifically enniatins B, B1 and beauvericin were detected with concentrations up to 4.04 μg/kg.
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dc.format.extent7
dc.identifier.citationMicrochemical Journal, 2023, Vol. 185 : 108192
dc.identifier.doihttps://doi.org/10.1016/j.microc.2022.108192
dc.identifier.eissn1095-9149
dc.identifier.issn0026-265X
dc.identifier.urihttp://hdl.handle.net/10201/168349
dc.languageeng
dc.publisherElsevier
dc.relationMinisterio de Cultura, Deporte y Turismo (MCIN) de España (Proyecto PID2021-123201NB-I00 financiado por MCIN/AEI/10.13039/501100011033/FEDER, UE) y la Comunidad Autónoma de la Región de Murcia (CARM, Fundación Séneca, Proyecto 21665/PDC/21).
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0026265X22010207
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMycotoxins
dc.subjectBioaccumulation
dc.subjectLiver
dc.subjectHuman
dc.subjectAnimals
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleBioaccumulation of mycotoxins in human forensic liver and animal liver samples using a green sample treatment
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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