Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum

Lorenzo Gutierrez, D.; Gómez Gil, L.; Guarro, J.; Roncero, M. I. G.; Capilla, J.; López Fernández, L.

Publication:
Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum

dc.contributor.author	Lorenzo Gutierrez, D.
dc.contributor.author	Gómez Gil, L.
dc.contributor.author	Guarro, J.
dc.contributor.author	Roncero, M. I. G.
dc.contributor.author	Capilla, J.
dc.contributor.author	López Fernández, L.
dc.contributor.department	Genética y Microbiología
dc.date.accessioned	2021-07-08T22:12:22Z
dc.date.available	2021-07-08T22:12:22Z
dc.description	© <2021>. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted Manuscript version of a Published Work that appeared in final form in VIRULENCE. To access the final edited and published work see DOI 10.1080/21505594.2020.1809324
dc.description.abstract	Cu is an essential trace element for cell growth and proliferation. However, excess of Cu accumulation leads to cellular toxicity. Thus, precise and tight regulation of Cu homeostasis processes, including transport, delivery, storage, detoxification, and efflux machineries, is required. Moreover, the maintenance of Cu homeostasis is critical for the survival and virulence of fungal pathogens. Cu homeostasis has been extensively studied in mammals, bacteria, and yeast, but it has not yet been well documented in filamentous fungi. In the present work, we investigated Cu tolerance in the filamentous fungus Fusarium oxysporum by analysing the Cu transporter coding gene crpF, previously studied in Aspergillus fumigatus. The expression studies demonstrated that crpF is upregulated in the presence of Cu and its deletion leads to severe sensitivity to low levels of CuSO4 in F. oxysporum. Targeted deletion of crpF did not significantly alter the resistance of the fungus to macrophage killing, nor its pathogenic behaviour on the tomato plants. However, the targeted deletion mutant ΔcrpF showed increased virulence in a murine model of systemic infection compared to wild-type strain (wt).
dc.format	application/pdf	es
dc.format.extent	15
dc.identifier.citation	Virulence, 2020, Vol. 11, Nº. 1, pp.:1108–1121
dc.identifier.doi	https://doi.org/10.1080/21505594.2020.1809324
dc.identifier.issn	2150-5594
dc.identifier.uri	http://hdl.handle.net/10201/110617
dc.language	eng	es
dc.relation.isreferencedby	ED_IDENTRADA=906
dc.rights	info:eu-repo/semantics/openAccess	*
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.title	Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es