Publication: Therapeutic effects of platelet derived growth factor overexpressed-mesenchymal stromal cells and sheets in canine skin wound healing model
Authors
Kim, Namyul ; Choi, Kyeong Uk ; Lee, Eunbee ; Lee, Seoyun ; Oh, Jiwon ; Kim, Woo keyoung ; Woo, Sang-Ho ; Kim, Dae-Yong ; Kim, Wan-Hee ; Kweon, Oh-kyeong
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-196
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info:eu-repo/semantics/article
Description
Abstract
Adipose-derived mesenchymal stromal cells
(Ad-MSCs) have excellent potential for skin wound
repair. Moreover, platelet-derived growth factor (PDGF)
has strong wound healing properties. The purpose of the
present study was to compare the healing effects of
PDGF-overexpressing canine allogeneic Ad-MSCs
(PDGF-MSCs) and their cell sheets (PDGF-CSs) as
compared to unexpressed Ad-MSCs (U-MSCs) and their
cell sheets (UCSs) in a cutaneous wound healing model
induced upon dogs. In in vitro study, the expression of
immunomodulatory and growth factors was assessed by
qRT-PCR. In in vivo study, cells and sheets were
transplanted into a square-shaped full-thickness (1.5×1.5
cm) skin defect model created in 12 dogs. After 5 and 10
days, wounds were harvested and evaluated
macroscopically and histopathologically. The qRT-PCR
results showed that the PDGF-B gene was significantly
upregulated (p<0.05) in PDGF-CS and PDGF-MSCs
groups. Upon gross analysis of the wound, all stromal
cells and their sheet groups showed accelerated (p<0.05)
cutaneous wound healing compared to the negative
control groups. As compared to U-MSCs and UCSs, the
PDGF-MSCs showed significant epithelization on days
5 and 10 of healing, whereas PDGF-CSs showed
improved epithelization only on day 10. In the
granulation tissue analysis, PDGF-CSs and UCSs
promoted more formation (p<0.05) of upper granulation
tissue, collagen, and activated fibroblasts than PDGF-
MSCs, and U-MSCs. Especially, the PDGF-CSs
presented the highest formation and maturation of
granulation tissue among all groups. All considered,
PDGF overexpressed stromal cells or cells sheets can
improve cutaneous wound healing in a canine model.
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Citation
Histology and Histopathology Vol. 35, nº7 (2020)
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