Publication: Histochemical localization of sialylated glycoconjugates with Tritrichomonas mobilensis lectin (TLM)
Loading...
Date
1996
Authors
Babál, P. ; Gardner, Jr., W.A.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
A new sialic acid-specific lectin from the
colonic parasite of squirrel monkeys Tritrichomonas
mobilensis (TML) was tested on human and mouse
tissues for histochemical staining properties. There were
no substantial differences in reactivity between frozen
and formalin fixed paraffin sections. TML staining was
blocked by preincubation with sialic acid or by sialidase
digestion. TMLIanti-TML antibody histochemistry was
identical with the TML-gold technique. The staining
pattern was not blood group dependent. TML stained
strongly the luminal membranes of normal vascular
endothelium as well as endothelial neoplasms.
Lymphatic vessels and capillaries of kidney glomeruli
and lung alveolar septi were negative or only slightly
positive. In parenchymatous organs luminal membrane
positivity was dominant, preferably of cells lining ducts.
Weak fine-granular cytoplasmic and basolateral
membrane staining was also observed. Umbrella cells in
transitional epithelium and basa1 layers of squamous
epithelia showed strong reactivity with cell membranes.
Mucin in respiratory epithelium was positive whereas
gastrointestinal mucins failed to stain uniformly.
Erythrocytes and most white blood cell types showed
distinct membrane positivity. Acetylation or alkaline Odeacetylation
of tissue sections did not substantially
change TML reactivity. Oxidation, however, completely
blocked TML staining except for respiratory epithelium
and colonic mucin.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.