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Genetic and molecular alterations in rhabdomyosarcoma: mRNA overexpression of MCL1 and MAP2K4 genes

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dc.contributor.authorPazzaglia, Lauraes
dc.contributor.authorChiechi, Antonellaes
dc.contributor.authorConti, Amalia
dc.contributor.authorGamberi, Gabriella
dc.contributor.authorMagagnoli, Giovanna
dc.contributor.authorNovello, Chiara
dc.contributor.authorMorandi, Luca
dc.contributor.authorPicci, Piero
dc.contributor.authorMercuri, Mario
dc.contributor.authorBenassi, M.S.
dc.date.accessioned2013-09-24T11:05:55Z
dc.date.available2013-09-24T11:05:55Z
dc.date.issued2009
dc.description.abstractRhabdomyosarcoma, the most common soft tissue sarcoma in childhood, belongs to the small round cell tumor family and is classified according to its histopathological features as embryonal, alveolar and pleomorphic. In this study we propose to explore genetic alterations involved in rhabdomyosarcoma tumorigenesis and assess the level of mRNA gene expression of controlling survival signalling pathways. For genetic and molecular analysis, array-based comparative genomic hybridization, combined with Real Time PCR using the comparative method, was performed on 14 primary well-characterized human primary rhabdomyosarcomas. Multiple changes affecting chromosome arms were detected in all cases, including gain or loss of specific regions harbouring cancer progression-associated genes. Evaluation of mRNA levels showed in the majority of cases overexpression of MCL1 and MAP2K4 genes, both involved in cell viability regulation. Our findings on rhabdomyosarcoma samples showed multiple copy number alterations in chromosome regions implicated in malignancy progression and indicated a strong expression of MAP2K4 and MCL1 genes, both involved in different biological functions of complicated signalling pathways.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/35965
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectGene expressiones
dc.subjectRhabdomyosarcomaes
dc.subject.other616 - Patología. Medicina clínica. Oncologíaes
dc.titleGenetic and molecular alterations in rhabdomyosarcoma: mRNA overexpression of MCL1 and MAP2K4 geneses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.24, nº1 (2009)