Publication: Immunohistochemical analyses on serum proteins in nephrons of protein-overload mice by in vivo cryotechnique
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Date
2007
Authors
Zhou, D.S. ; Ohno, N. ; Terada, N. ; Li, Z. ; Morita, H. ; Inui, K. ; Yoshimura, A. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Immunohistochemical analyses on local
distributions of serum proteins in living mouse kidneys
are usually difficult to examine with conventional
preparation methods. By using our “in vivo
cryotechnique” combined with freeze-substitution, we
have checked immunolocalizations of the serum proteins
in nephrons of bovine serum albumin (BSA)-overload
mice, and compared them with those obtained by the
conventional preparation methods. In two days of daily
BSA-injected mice, the immunolocalization of BSA
could be observed in Bowman’s space and urinary
tubules with their overt proteinuria, where another
endogenous mouse albumin was similarly
immunolocalized. The leakage of BSA and mouse
albumin in Bowman’s space and their reabsorption into
proximal tubules were detected in 55% of nephrons,
where no leakage of immunoglobulin G1 (IgG1) was
detected. However, the leakage of IgG1, in addition to
BSA and mouse albumin, was detected in the other nephrons. By carefully examining immunolocalizations
of BSA and IgG1, they were obviously different from
those obtained by the conventional preparation methods
without normal blood circulation into the kidneys. The
immunolocalizations of both BSA and mouse serum
proteins could be directly analyzed with the “in vivo
cryotechnique”, suggesting that functional damage to
glomerular filtration barriers are different at early stages
of the BSA-overload mouse model, depending on each
nephron of living mice.
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