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Evaluation of a commercial double-recognition ELISA for caprine tuberculosis diagnosis: sensitivity, specificity, and correlation to macroscopic and microscopic lesions

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dc.contributor.authorSalinas Lorente, Jesús
dc.contributor.authorDel Río, Laura
dc.contributor.authorBuendía Marín, Antonio Julián
dc.contributor.authorNavarro Cámara, José Antonio
dc.contributor.authorOrtega Hernández, Nieves
dc.contributor.authorSánchez Campillo, Joaquín
dc.contributor.departmentSanidad Animal
dc.date.accessioned2024-12-16T08:13:43Z
dc.date.available2024-12-16T08:13:43Z
dc.date.issued2022-10-04
dc.description© 2022 Elsevier Ltd. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/10.1016/j.rvsc.2022.09.034
dc.description.abstractAnte-mortem diagnosis of caprine tuberculosis, as in other ruminant species, is a complex process. Therefore, it is sometimes necessary to apply a combination of methods that increases the sensitivity of diagnosis while maintaining adequate specificity. In the present study, we analysed the use of ELISA based on a double-recognition methodology for the diagnosis of goat tuberculosis. Serum samples from animals with tuberculosis, confirmed by histopathological studies and presence of Ziehl-Neelsen-positive bacteria in the lesions, were used to assess sensitivity. Sera from flocks without tuberculosis were used to assess diagnostic specificity. The relationship between ELISA results and the type of tuberculous lesions, both macroscopic and microscopic, was analysed. The sensitivity and specificity of ELISA test were 69.9% and 97.9%, respectively. Sensitivity was higher in animals with macroscopic lesions, indicating more advanced forms of the disease. Similarly, a higher sensitivity was found in animals with microscopic lesions associated with active tuberculosis, such as cavitary lesions.es
dc.formatapplication/pdfes
dc.format.extent6es
dc.identifier.doihttps://doi.org/10.1016/j.rvsc.2022.09.034
dc.identifier.eisbnResearch in Veterinary Science 152 (2022) 618-623es
dc.identifier.issnPrint: 0034-5288
dc.identifier.issnElectronic: 1532-2661
dc.identifier.urihttp://hdl.handle.net/10201/147480
dc.languageenges
dc.publisherElsevieres
dc.relationSin financiación externa a la Universidades
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0034528822003149?ref=pdf_download&fr=RR-9&rr=8f25ff9d4d2069e5es
dc.rightsinfo:eu-repo/semantics/restrictedAccesses
dc.subjectGoates
dc.subjectTuberculosises
dc.subjectDiagnosises
dc.subjectELISAes
dc.subjectLesionses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología::616.9 - Enfermedades infecciosas y contagiosas. Fiebreses
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiologíaes
dc.titleEvaluation of a commercial double-recognition ELISA for caprine tuberculosis diagnosis: sensitivity, specificity, and correlation to macroscopic and microscopic lesionses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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