Determination of Chitin and protein contents during the isolation of Chitin from shrimp waste

Díaz Rojas, Erika I.; Argüelles Monal, Waldo M.; Higuera Ciapara, Inocencio; Hernández, Javier; Lizardi Mendoza, Jaime; Goycoolea Valencia,  Francisco Martín

Publication:
Determination of Chitin and protein contents during the isolation of Chitin from shrimp waste

dc.contributor.author	Díaz Rojas, Erika I.
dc.contributor.author	Argüelles Monal, Waldo M.
dc.contributor.author	Higuera Ciapara, Inocencio
dc.contributor.author	Hernández, Javier
dc.contributor.author	Lizardi Mendoza, Jaime
dc.contributor.author	Goycoolea Valencia, Francisco Martín
dc.contributor.department	Biología Celular e Histología
dc.date.accessioned	2025-01-29T07:35:46Z
dc.date.available	2025-01-29T07:35:46Z
dc.date.issued	2006-05-09
dc.description	© 2006 WILEY-VCH Verlag GmbH & Co. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted Manuscript version of a Published Work that appeared in final form in Macromolecular Bioscience. To access the final edited and published work see https://doi.org/10.1002/mabi.200500233
dc.description.abstract	Accurate determination of chitin and protein contents in crustacean biomass and the intermediate products during the industrial isolation of chitin cannot be made directly from the total nitrogen content, unless the appropriate corrections are applied. This method, however, is affected by the presence of other nitrogen-containing chemical species that are formed endogenously or by the action of microorganisms during the handling of the sample. Therefore, an alternative rapid method to estimate the contents of these components can be very useful both in research and in various fields of application. An original method has been developed to address this problem. The method consists of the development of a set of equations based on the stoichiometric contents of nitrogen of chitin and protein whereby the amounts of each component can be estimated from the value of the total nitrogen content, provided the rest of the proximate composition of the sample is accurately known. In order to validate the procedure, a set of model mixtures of pure chitin and protein concentrate in the solid state, both extracted from shrimp head waste, are used. Excellent agreement between the predicted and real values of chitin and protein are obtained (R2=0.98, slope=0.90). When the proposed method is tested in the analysis of real samples obtained from five different processing protocols of pretreatment of raw shrimp head, it is found that in general, the values of protein and chitin contents throughout the various stages of the process vary as expected.	es
dc.format	application/pdf	es
dc.format.extent	8	es
dc.identifier.citation	Macromolecular Bioscience, 2006, Vol. 6, Issue 5, pp. 340-347
dc.identifier.doi	https://doi.org/10.1002/mabi.200500233
dc.identifier.issn	Print: 1616-5187
dc.identifier.issn	Electronic: 1616-5195
dc.identifier.uri	http://hdl.handle.net/10201/149580
dc.language	eng	es
dc.publisher	Wiley
dc.relation	Ambito del proyecto: Europeo. Unión Europea. Convocatoria ALFA. Proyecto "Polylife" Ref. II-0259-FA-FDC. Two scholarship grants from CONACYT to EIDR	es
dc.relation.publisherversion	https://onlinelibrary.wiley.com/doi/10.1002/mabi.200500233
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Chitin	es
dc.subject	Protein
dc.subject	Proximate analysis
dc.subject	Shrimp head waste
dc.subject	Stoichiometric nitrogen
dc.subject.other	CDU::6 - Ciencias aplicadas::60 - Cuestiones generales de las ciencias aplicadas	es
dc.title	Determination of Chitin and protein contents during the isolation of Chitin from shrimp waste	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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