Publication: Measurable cytokine concentrations in pig seminal plasma are modified by semen handling and storage
Authors
Padilla, Lorena ; Barranco, Isabel ; Parrilla, Inmaculada ; Lucas, Xiomara ; Rodríguez-Martínez, Heriberto ; Roca, Jordi
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Publisher
MDPI
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DOI
https://doi.org/10.3390/biology9090276
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info:eu-repo/semantics/article
Description
© 2020 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published version of a Published Work that appeared in final form in Biology. To access the final edited and published work see https://doi.org/10.3390/biology9090276
Abstract
Sample handling and storing are critical steps for the reliable measurement of circulating biomolecules in biological fluids. This study evaluates how cytokine measurements in pig seminal plasma (SP) vary depending on semen handling and SP storage. Thirteen cytokines (GM-CSF, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFα) were measured using Luminex xMAP® technology in individual seminal plasma (SP) samples (n = 62) from healthy breeding boars. Three separate experiments explored the delay (2 h and 24 h) in SP collection after ejaculation (Experiment 1) and SP storage, either short-term (5 °C, -20 °C and -80 °C for 72 h, Experiment 2) or long-term (at -20 °C and -80 °C for two months, Experiment 3), before analysis. Levels in fresh SP-samples were used as baseline control values. Delays in SP harvesting of up to 24 h did not substantially impact SP cytokine measurements. Some cytokines showed instability in stored SP samples, mainly in long-term storage. Ideally, cytokines in pig SP should be measured in fresh samples harvested within 24 h after ejaculation. If storage of SP is imperative, storage conditions should be adjusted for each cytokine.
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Citation
Biology, 2019, Vol. 9 (9): 276
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Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by/4.0/