Publication:
Differential functional regulation of protein kinase C (PKC) orthologs in fission yeast.

Simple item page
dc.contributor.authorMadrid, M.
dc.contributor.authorVázquez-Marín, B.
dc.contributor.authorSoto, T.
dc.contributor.authorFranco, A.
dc.contributor.authorGómez-Gil, E.
dc.contributor.authorVicente-Soler, J.
dc.contributor.authorGacto, M.
dc.contributor.authorPérez, P.
dc.contributor.authorCansado Vizoso, José
dc.contributor.departmentGenética y Microbiología
dc.date.accessioned2021-03-16T12:33:56Z
dc.date.available2021-03-16T12:33:56Z
dc.date.created2017
dc.date.issued2017-07
dc.description.abstractThe two PKC orthologs Pck1 and Pck2 in the fission yeast Schizosaccharomyces pombe operate in a redundant fashion to control essential functions, including morphogenesis and cell wall biosynthesis, as well as the activity of the cell integrity pathway (CIP) and its core element the MAPK Pmk1. We show here that despite the strong structural similarity and functional redundancy of these two enzymes, the mechanisms regulating their maturation, activation, and stabilization have a remarkably distinct biological impact on both kinases. We found that in contrast to Pck2, putative in vivo phosphorylation of Pck1 within the conserved activation loop, turn and hydrophobic motifs is essential for Pck1 stability and biological functions. Constitutive Pck activation promoted dephosphorylation and destabilization of Pck2, while it enhanced Pck1 levels to interfere with proper downstream signaling to the CIP via Pck2. Importantly, whereas catalytic activity was essential for Pck1 function, Pck2 remained partially functional independently of its catalytic activity. Our findings suggest that early divergence from a common ancestor in fission yeast involved important changes in the mechanisms regulating catalytic activation and stability of PKC family members to allow for flexible and dynamic control of downstream functions, including MAPK signaling.es
dc.formatapplication/pdfes
dc.format.extent13es
dc.identifier.citationThe Journal of biological chemistry. 2017; 292 (27): 11374-11387
dc.identifier.doi10.1074/jbc.M117.786087
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/10201/105025
dc.languageenges
dc.publisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INCes
dc.relationTitulo: Interacción entre la ruta de MAP quinasas de integridad celular y otras vías de señalización intracelular en la levadura con fisión. Ministerio de Economía y Competitivdad. Plan Nacional Convocatoria de Excelencia, 2014. BFU2014-52828-P. Título: Activación diferencial de isoformas de PKC y papel regulador de la ruta de MAP quinasas de integridad celular en S. pombe. Fundación Séneca. Ayudas a la realización de proyectos para el desarrollo de la investigación científica y técnica por grupos competitivos 2014. 19346/PI/14es
dc.relation.isreplacedbyhttps://www.sciencedirect.com/science/article/pii/S0021925820370083?via%3Dihubes
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0021925820370083?via%3Dihubes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMAPKes
dc.subjectPKCes
dc.subjectRho (Rho GTPase)es
dc.subjectSchizosaccharomyces pombees
dc.subjectSignal Transductiones
dc.subjectStress Responsees
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::579 - Microbiologíaes
dc.titleDifferential functional regulation of protein kinase C (PKC) orthologs in fission yeast.es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Manuscript JBC-2017-786087.pdf
Size:
3.82 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.39 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos