Publication:
Synergistic antimicrobial effect of photodynamic therapy and chitosan on the titanium-adherent biofilms of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa: An in vitro study

Simple item page
dc.contributor.authorCamacho Alonso, Fabio
dc.contributor.authorSalinas, Jesús
dc.contributor.authorSánchez Siles, Mariano
dc.contributor.authorPato Mourelo, Jesús
dc.contributor.authorCotrina Veizaga, Brian Davis
dc.contributor.authorOrtega Hernández, Nieves
dc.contributor.departmentDermatología, Estomatología, Radiología y Medicina Física
dc.contributor.departmentSanidad Animal
dc.date.accessioned2024-01-31T18:29:34Z
dc.date.available2024-01-31T18:29:34Z
dc.date.issued2022
dc.description.abstractBackground: To date, no studies on the combined use of photodynamic therapy (PDT) and chitosan against peri-implantitis have been published. The aim of this study was to evaluate the possible synergistic antimicrobial effect of PDT and chitosan on the titanium-adherent biofilms of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Methods: A total of 60 titanium discs were included in this study. The discs were randomized into three bacterial contaminations (n = 20 discs per bacterium). After being cultured (incubated for 48 hours) they were randomized again into four different disinfection modalities (n = 5 discs per treatment): control (without treatment), PDT, chitosan 3 mg/mL, and PDT + chitosan 3 mg/mL. After the treatments, the colony forming units (CFU) were measured to determine antimicrobial effects, and field emission scanning electron microscopy (FESEM) was used to study cell morphology and titanium adherence. Results: For all the evaluated bacteria and all the variables studied the order from highest to lowest antimicrobial effectiveness was: PDT + chitosan 3 mg/mL > chitosan 3 mg/mL > PDT > control. Although, all disinfection methods were significantly effective when compared to control, the combined treatment of PDT + chitosan 3 mg/mL had the greatest antimicrobial effect against the three studied bacteria. Conclusions: The combination of PDT and chitosan has a synergistic antimicrobial effect against the bacteria S. aureus, E. coli, and P. aeruginosa, all closely related to peri-implantitis. However, further in vivo studies are needed because this study provides data based on an in vitro scenario that might not be extrapolated to patients with peri-implantitis.es
dc.embargo.terms1-ene-2999
dc.formatapplication/pdfes
dc.format.extent12es
dc.identifier.citationJournal of Periodontology 2022 93(6):e104-e115
dc.identifier.doihttps://doi.org/10.1002/JPER.21-0306
dc.identifier.issn0022-3492
dc.identifier.urihttp://hdl.handle.net/10201/138325
dc.languageenges
dc.publisherWileyes
dc.relationSin financiación externa a la Universidades
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectChitosanes
dc.subjectEscherichia colies
dc.subjectPhototherapyes
dc.subjectPseudomonas aeruginosaes
dc.subjectStaphylococcus aureuses
dc.subject.otherCDU::5 - Ciencias puras y naturaleses
dc.titleSynergistic antimicrobial effect of photodynamic therapy and chitosan on the titanium-adherent biofilms of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa: An in vitro studyes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication58439903-59c1-4eaf-9b81-9633304c3e15
relation.isAuthorOfPublication.latestForDiscovery58439903-59c1-4eaf-9b81-9633304c3e15
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Journal of Periodontology - 2021 - Camacho‐Alonso - Synergistic antimicrobial effect of photodynamic therapy and chitosan.2022A (30).pdf
Size:
1.5 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos