Publication: Photobiomodulation therapy increases collagen II after tendon experimental injury
Authors
Akamatsu, Flávia Emi ; Teodoro, Walcy Rosolia ; Itezerote, Ana Maria ; Ramos da Silveira, Lizandre Keren ; Saleh, Samir ; Real Martinez, Carlos Augusto ; Lima Ribeiro, Marcelo ; Aires Pereira, José ; Hojaij, Flávio ; Andrade, Mauro ; Jacomo, Alfredo Luiz
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-330
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info:eu-repo/semantics/article
Description
Abstract
A tendon is a mechanosensitive tissue that
transmits muscle-derived forces to bones.
Photobiomodulation (PBM), also known as low-level
laser therapy (LLLT), has been used in therapeutic
approaches in tendon lesions, but uncertainties regarding
its mechanisms of action have prevented its widespread
use. We investigated the response of PBM therapy in
experimental lesions of the Achilles tendon in rats.
Thirty adult male Wistar rats weighing 250 to 300 g
were surgically submitted to bilateral partial transverse
section of the Achilles tendon. The right tendon was
treated with PBM, whereas the left tendon served as a
control. On the third postoperative day, the rats were
divided into three experimental groups consisting of ten
rats each, which were treated with PBM (Konf, Aculas -
HB 750), 780 nm and 80 mW for 20 seconds, three
times/week for 7, 14 and 28 days. The rats were
sacrificed at the end of the therapeutic time period. The
Sca-1 was examined by immunohistochemistry and
histomorphometry, and COLA1, COLA2 and COLA3
gene expression was examined by qRT-PCR. COLA2
gene expression was higher in PBM treated tendons than
in the control group. The histomorphometric analysis
coincided with increased number of mesenchymal cells,
characterized by Sca-1 expression in the lesion region
(p<0.001). PBM effectively interferes in tendon tissue
repair after injury by stimulating mesenchymal cell
proliferation and the synthesis of collagen type II, which
is suggested to provide structural support to the
interstitial tissues during the healing process of the
Achilles tendon. Further studies are needed to confirm
the role of PBM in tendon healing.
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Citation
Histology and Histopathology Vol. 36, nº6 (2021)
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