Publication: Isoflurane post-conditioning stimulates the
proliferative phase of myocardial recovery in an
ischemia-reperfusion model of heart injury in rats
Authors
Agnić, Ivan ; Vukojevic, Katarina ; Saraga-Babić, Mirna ; Filipović, Natalija ; Grković, Ivica
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Summary. The application of isoflurane in a postconditioning
manner, during early reperfusion following
a period of coronary occlusion, has numerous beneficial
effects on the ischemic myocardium, including reduction
of infarct size. It does so by stimulating a sequence of
well studied anti-apoptotic pro-survival mechanisms in a
similar manner to various ‘ischemic’ pre-/postconditioning
approaches which achieve their cardio
protective effects in both laboratory and clinical
situations. Proliferation of newly formed blood vessels,
resulting in formation of highly vascularized granulation
tissue, is an essential stage of infarct healing. It can be
evaluated by detecting various angiogenic factors,
including vascular endothelial growth factor (VEGF)
and platelet endothelial cell adhesion molecule-1
(PECAM-1/CD31) or by quantification of expression of
vascular smooth muscle progenitors, such as Nestin.
Expression of these three markers was used to evaluate
the effect of early isoflurane post-conditioning in
ischemia-reperfusion type cardiac injury. A large
reduction in infarct size (59.3% of control), and marked
increase of expression of VEGF (43.4%), PECAM1/CD31
(136%) and Nestin (77.9%) was found in
experimental animals when compared to control animals
that did not receive isoflurane treatment. Hence, based
on our results, we can emphasize two morphologically
detectable benefits of isoflurane post-conditioning: a
marked reduction in infarct size and much better
organization/vascularization of necrotic tissue.
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Citation
Histology and Histopathology, vol. 29, nº1, (2014)
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