Publication: Supplementation of University of Wisconsin solution with Nitroglycerin and Nicorandil in long-term myocardial preservation: effects on the
oxidative state, endothelial function and morphology
Loading...
Date
2009
Authors
Álvarez-Ayuso, Lourdes ; García Gómez-Heras, Soledad ; Jorge, Eduardo ; Guardiola, José M. ; Torralba, Amalia ; Millán, Isabel ; Roda, Jorge R. ; Calero, Patricia ; García-Poblete, Eduardo ; Fernández-García, Héctor
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The purpose of this study was to assess the
effects of the addition of Nitroglycerin or Nicorandil to
University of Wisconsin solution in long-term
myocardial preservation. In a model of heterotopic heart
transplantation in pigs, the donor heart was preserved for
24 hours by means of continuous perfusion in this
solution, in the presence or absence of these drugs.
During this period, the oxygenation and pH of the
solution were measured, as were lactate concentrations
and enzyme release. At regular intervals following
reperfusion we measured the concentrations of enzymes,
antioxidants, glutathione peroxidase, glutathione
reductase, malondialdehyde, endothelin and nitrite, and,
two hours later, samples of both ventricles were taken
for a morphological study. In the treated groups there
was a higher lactate production during preservation and,
during reperfusion, the signs of contracture and the
elevation of enzyme levels were more marked than in
the untreated groups. In contrast, the glutathione
reductase concentrations did not decrease during the first
phase of reperfusion and were directly correlated with
those of antioxidants, endothelin levels increased less
than in the untreated groups and, in the case of
nitroglycerin, the nitrite concentration was significantly
greater than in the remaining groups. We conclude that
nitroglycerin and nicorandil improved the oxidative state
and endothelial function and did not produce substantial
morphological changes, but increased cell necrosis and
contracture, possibly due to the duration of ischemia.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.