Publication:
Decreased expression of p16 in ovarian cancers represents an unfavourable prognostic factor

Simple item page
dc.contributor.authorSurowiak, Paweles
dc.contributor.authorMaterna, Verenaes
dc.contributor.authorMaciejczyk, Adam
dc.contributor.authorPudelko, Marek
dc.contributor.authorSuchocki, Slawomir
dc.contributor.authorKedzia, Witold
dc.contributor.authorNowak-Markwitz, Ewa
dc.contributor.authorDumanska, Malgorzata
dc.contributor.authorSpaczynski, Marek
dc.contributor.authorZabel, M.
dc.contributor.authorDietel, Manfred
dc.contributor.authorLage, Hermann
dc.date.accessioned2013-01-22T10:01:03Z
dc.date.available2013-01-22T10:01:03Z
dc.date.issued2008
dc.description.abstractDecreased expression of p16 may result from hypermethylation of the promoter or from deletion of the gene. It can lead to intensified proliferation of neoplastic cells and to cytostatic drug resistance. The study was aimed at the examination of prognostic value of p16 expression in relation to Ki67 and caspase-3 in ovarian cancers using immunohistochemistry. The immunohistochemical studies were performed on 73 paraffinembedded samples of ovarian cancers from 43 patients and samples from 6 healthy ovaries. We have used monoclonal antibodies against p16. ABC method and DAB were used for antigens visualisation. The intensity of the immunohistochemical reactions was appraised using the semi-quantitative IRS scale. In healthy ovaries we have shown strong reaction in the nuclei of surface epithelium. In the case of studied ovarian cancers, the reaction of a nuclear and cytoplasmic localization was obtained. The mean overall immunoreactivity score of nuclear p16 expression amounted to 5.30±3.44 SD in primary laparotomy material and 6.61±4.34 SD in secondary cytoreduction material. Statistical analysis demonstrated that lower p16 expression was typical of the younger patients and the patients who died. Kaplan- Meier’s analysis proved that lower expression of p16 was characteristic of cases with shorter overall survival. In the present study we have demonstrated that lowered p16 expression represented an unfavourable prognostic index in ovarian cancer. Lowered p16 expression was also typical for chemotherapy-resistant ceases (cases of lower caspase-3 and higher Ki67 at secondary cytoreduction expression).es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/29800
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectOvarian canceres
dc.subjectImmunohistochemistryes
dc.subject.other616 - Patología. Medicina clínica. Oncologíaes
dc.titleDecreased expression of p16 in ovarian cancers represents an unfavourable prognostic factores
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Decreased expression of p16 in ovarian cancers represents an unfavourable prognostic factor.pdf
Size:
749.34 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Histology and histopathology Vol.23, nº5 (2008)