Publication: Immunohistochemical localization
of galectins-1 and -3 and monitoring of
tissue galectin-binding sites during tubular
regeneration after renal ischemia reperfusion in the rat
Authors
Vansthertem, David ; Cludts, Stéphanie ; Nonclercq, Denis ; Gossiaux, Annabel ; Saussez, Sven ; Legrand, Alexandre ; Gabius, H.J. ; Toubeau, Gérard
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Endogenous lectins act as effectors of
cellular activities such as growth regulation, migration
and adhesion. In this study, we report the histochemical
detection of galectins and their binding sites in rat
kidneys after ischemic injury (35 min) with regard to
renal regeneration. In this context, we have shown in a
previous publication (Vansthertem et al., 2008) that
extrarenal cells (CD44+, vimentin +) could be involved
in this process of tubular restoration. In controls,
galectin-1 is expressed by fusiform-shaped cells within
cortical and medullar interstitium. Two days after
ischemia, the number of positive interstitial cells
increased temporarily within OSOM in the vicinity of
altered tubules to later reach control level. After
ischemia, we identified a population of galectin-3 (+),
CD44 (+), and vimentin (+) interstitial round cells
located in the outer stripe of outer medulla (OSOM) in
the vicinity of necrotic tubules, but also in the lumen of
adjacent blood vessels. The immunocytochemical
characteristics of theses cells, along with their
distribution within OSOM, suggest the involvement of a
unique cell population during kidney regeneration. On
the other hand, the distribution and density of binding
sites for galectins within OSOM were not modified after
ischemia and remained similar to controls. Altogether,
our observations suggest that galectin-3 may be involved
in the complex process of kidney regeneration following
ischemia/reperfusion injury.
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