Publication: Structural patterns of swine ileal mucosa following L-glutamine and
nucleotide administration during the weaning
period. An histochemical and histometrical study
Authors
Domeneghini, C. ; Di Giancamillo, A. ; Savoini, G. ; Paratte, R. ; Bontempo, V. ; Dell’Orto, V.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Dietary supplementations with L-glutamine
and/or nucleotides were screened for their effects on
intestinal mucosa in 16 female weaning piglets. The
animals were transported to the university’s facilities 24
hours after weaning. They were grouped four to a pen in
controlled environmental conditions and fed one of the
following four diets for 28 days: control diet (C);
C+0.5% L-glutamine (G); C+0.05% “nucleotides” (N);
and C+0.5 % L-glutamine+0.05% “nucleotides” (GN).
Individual body weights and feed intake per group were
recorded at the beginning and the end of the study as
well as weekly during it. There were no significant
performance differences among the groups. After 28
days the animals were slaughtered and the distal ileum
and liver were examined histologically. Antiproliferating
cell nuclear antigen (PCNA) as well as antihuman
macrophage immunostaining, and a modified
TdT-mediated dUTP nick-end labeling technique
(TUNEL) were performed, and intraepithelial
lymphocyte percentage was evaluated to assess morphofunctional
aspects of the ileum. Histometry was
performed by assessing cell indices and counts of
immuno-reactive structures.
Feeding G and/or N resulted in an increase in villi
(V) height, crypt (C) depth, and a decrease in V:C ratio P<0.01). In addition, feeding G and/or N resulted in an
increase in mitotic mucosal cells (M), and a decrease in
apoptotic mucosal cells (A), thus decreasing the A:M
index (P<0.01). The percentages of mucosal
macrophages were greater in G and/or N groups
(P<.001) than in control piglets, and similarly among the
groups the percentages of intraepithelial lymphocytes
varied (P<0.01).
Our data showed that the diet supplementation with G and/or N had positive effects on some morphofunctional
characteristics of piglet ileal mucosa. These
ameliorative effects may potentially be linked to a good
responsiveness of piglets to a stressful period, like a
precocious weaning is in this species.
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