Publication: Preferential expression of cystein-rich
secretory protein-3 (CRISP-3) in chronic pancreatitis
Authors
Liao, Q. ; Kleeff, Jörg ; Xiao, Y. ; Guweidhi, A. ; Schambony, A. ; Töpfer-Petersen, E. ; Zimmermann, Astrid ; Büchler, M.W. ; Friess, Helmut
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Background: Chronic pancreatitis (CP) is a
progressive inflammatory process resulting in exocrine
and endocrine pancreatic insufficiency in advanced
stages. Cysteine-rich secretory protein (CRISP-3) has
been identified as a defense-associated molecule with
predominant expression in the salivary gland, pancreas
and prostate. Aims: In this study, we investigated
CRISP-3 expression in normal pancreatic tissues,
chronic pancreatitis tissues, pancreatic cancer tissues and
pancreatic cancer cell lines, as well as in other
gastrointestinal organs. Materials and Methods: 15
normal pancreatic tissues, 14 chronic pancreatitis tissues
and 14 pancreatic cancer tissues as well as three
pancreatic cancer cell lines were analyzed. Moreover,
hepatocellular carcinoma and esophageal, stomach and
colon cancers were also analyzed together with the
corresponding normal controls. Results: CRISP-3 was
expressed at moderate to high levels in chronic
pancreatitis tissues and at moderate levels in pancreatic
cancer tissues but at low levels in normal pancreatic
tissues, and was absent in three pancreatic cancer cell
lines. CRISP-3 expression was below the level of
detection in all cancerous gastrointestinal tissues and in
all normal tissues except 2 of 16 colon tissue samples.
CRISP-3 mRNA signals and immunoreactivity were
strongly present in the cytoplasm of degenerating acinar
cells and in small proliferating ductal cells in CP tissues
and CP-like lesions in pancreatic cancer tissues. In
contrast, CRISP-3 expression was weak to absent in the
cytoplasm of cancer cells as well as in acinar cells and
ductal cells in pancreatic cancer tissues and normal
pancreatic tissues. Conclusion: These results reveal that
the distribution of CRISP-3 in gastrointestinal tissues is
predominantly in the pancreas. High levels of CRISP-3
in acinar cells dedifferentiating into small proliferating
ductal cells in CP and CP-like lesions in pancreatic cancer suggests a role of this molecule in the
pathophysiology of CP.
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