Publication: Bronchial-associated lymphoid tissue (BALT) response to airway challenge with cigarette smoke,bovine antigen and anti-pulmonary serum
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Date
1992
Authors
Escolar Castellón, J.de D. ; Escolar castellón, A. ; Roche Roche, P.A. ; Miñana Amada, C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The bronchial-associated lymphoid tissue
(BALT) is a lymphoepithelial organ, related to the
immune defence of the lung and to alveolar clearance,
which changes size in certain states of disease.
Changes in the size of BALT were quantified and
compared, and Spearman's test was used to test the
relation with the bronchial epithelium.
A total of 180 rats were used, divided into 6 groups
of 30 as follows: 1) untreated controls; 2) exposed to
cigarette smoke for two months; 3) treated with antipulmonary
serum three doses daily over five days; 4)
exposed to cigarette smoke and treated with antipulmonary
serum; 5) sensitized with bovine albumin
and exposed to an environment containing this antigen
for two months; 6) exposed to cigarette smoke and
bovine albumin.
The lungs were processed for histological study,
and were stained with the PAS-Alcian blue method.
The main left bronchi BALT was studied, and the
following were quantified: Lymphatic area (LA), as a
percentage of the lung surface occupied by BALT; the
flat epithelium (FEp), as the length of bronchial
epithelium anatomically related to LA, whose cells
tend to adopt a flat shape; the Contact epithelium
(Cep), as the length of bronchial epithelium which is
in direct contact with the LA. A percentage count of
bronchial cells was made in the following
classifications: globet cells; globet cells stained with
the PAS-Alcian blue method; flat cells;
lymphoepithelium cells; columnar cells; and bronchial
epithelium cells excluding the above two cell types.
The most outstanding findings were that, in
comparison with the controls, the FEp and CEp had
increased in al1 the treated animals, and the CEp of
the group treated with bovine albumin + cigarette
smoke was the only one in which the difference was
Offprint requests to: Dr. D. Juan de Dios Escolar Castellón,
Facultad de Medicina, C./ Domingo Miral s/n, 50009 Zaragoza,
Spain
not significant (p > 0.05). The lymphatic area
increased significantly (p < 0.001) in the groups
treated with cigarette smoke, anti-pulmonary serum
and cigarette smoke + anti-pulmonary serum, and
decreased (p > 0.05) in the one treated with bovine
albumin. Regression analysis gave good indices in
general (Rho > 0.06), the exception being the group
treated with cigarette smoke + ALS, in the relations
CEpJFEp and CEpILA (Rho < 0.06). The globet ceiis
decreased significantly in al1 the treated animals;
however, the correlation between globet cells and the
other variables did not give good indices (Rho >
0.06).
Therefore, it is deduced that BALT can change
shape in different conditions, FEp being the variable
which increases most. The fa11 in globet ceiis is
associated exclusively with the substances which were
administered, since no relation was found between
these cells and the morphological changes in the
BALT.
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