Publication: Tensile strain promotes osteogenic differentiation of bone marrow mesenchymal stem cells through upregulating lncRNA-MEG3
Authors
Zhu, Guozheng ; Zeng, Canjun ; Qian, Yuepeng ; Yuan, Song ; Ye, Zelin ; Zhao, Shanwen ; L, Runguang
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-365
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info:eu-repo/semantics/article
Description
Abstract
Background. With the aging of the
population, osteoporosis is becoming more and more
common. This progressive bone disease increases the
risk of fractures and pain and causes serious harm to
people's health and quality of life. Several studies,
including our previous studies, confirmed that tensile
strain can promote bone marrow mesenchymal stem cell
(BMSC) osteogenic differentiation in vitro. In this study,
we further explored the mechanism by which tensile
strain regulates BMSC differentiation.
Methods. A device designed by our group was used
to apply tensile strain to BMSCs to study the effects of
tensile strain on their differentiation. LncRNA-MEG3
overexpression and silencing models of BMSCs were
constructed by lentivirus transfection to study the
involvement of lncRNA-MEG3. We assessed osteogenic
differentiation of BMSCs by alkaline phosphatase (ALP)
staining and the expression of Runx2 mRNA and BMP2
mRNA, while adipogenic differentiation was evaluated
by oil red staining and the expression of PPARγ mRNA
and C/EBPα mRNA.
Results. We demonstrated that proper tensile strain
can promote osteogenic differentiation of BMSCs while
inhibiting differentiation into adipocytes, and
simultaneously promote the expression of lncRNAMEG3. The overexpression of lncRNA-MEG3 further
promotes osteogenic differentiation of stressed BMSCs
and inhibits expression of miR-140-5p; the knockdown
of lncRNA-MEG3 induces the opposite effects.
Conclusion. Appropriate mechanical stimulation can
inhibit the expression of miR-140-5p by promoting
lncRNA-MEG3 expression, thereby promoting the
osteogenic differentiation of BMSCs. Our results
provide a theoretical basis for physical exercise to
improve the prevention and treatment of osteoporosis
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Citation
Histology and Histopathology Vol. 36, nº9 (2021)
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