Publication: Development of immune complex trapping: experimental study of lymphoid follicles and germinal centers newly induced by exogenous stimulants in mouse popliteal lymph nodes
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Date
1999
Authors
Horie, K. ; Chen, D. ; Hoshi, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The development of immune complex
trapping in newly-induced lymphoid follicles of draining
popliteal lymph nodes was investigated in young adult
mice, which had been given bilateral injection of
hemocyanin (KLH) or phytohemagglutinin (PHA), each
absorbed onto alumina. HRP-anti-HRP immune complex
was injected into the footpad 1 day before sacrifice.
Using three series of semi-serial cryostat sections
prepared from each popliteal node, the number of
lymphoid follicles in each node was counted, and
follicular localization of the in vivo injected and in vitro
applied immune complexes in each follicle was
determined. By day 5, a large germinal center had
developed within each preexisting follicle. A large
number of 'new' secondary follicles, each containing a
small PNA-positive germinal center, appeared outside
pre-existing follicles, from day 5 through day 11 in
KLH-treated nodes, and from day 7 through day 14 in
PHA-treated nodes. Shortly after their appearance, new
secondary follicles showed no in vitro or in vivo
trapping, but subsequently, many of the new follicles
began to display in vitro trapping, at first weakly but
later intensely. Occurrence of in vivo trapping in new
follicles took some time and was first recognized when
new follicles showed intense in vitro trapping. At day 21
or 25, many of the new follicles showed both in vitro
and in vivo trapping. It was concluded that in lymph
nodes treated with a stimulant, secondary follicles containing germinal centers can be formed de novo in
the extrafollicular zone where the follicular trapping
microenvironment is absent, but subsequently the microenvironment
capable of trapping immune complexes
develops at the site of formation of new follicles.
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