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Histochemical and ultrastructural study of skeletal muscle in patients with sepsis and multiple organ failure syndrome (MOFS)

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dc.contributor.authorDíaz, N. L.
dc.contributor.authorFinol, H. J.
dc.contributor.authorTorres, S. H.
dc.contributor.authorZambrano, C. I.
dc.contributor.authorAdjounian, H.
dc.date.accessioned2020-05-22T15:26:09Z
dc.date.available2020-05-22T15:26:09Z
dc.date.issued1998
dc.description.abstractMuscle biopsies for histochemical and ultrastuctural analysis were obtained from seven critically ill patients admitted to the Intensive Care Unit of the "Domingo Luciani" Hospital, Caracas, Venezuela. The sample included two patients with sepsis of abdominal origin, and five that presented sepsis/MOFS, with renal, hepatic, and respiratory disturbances and muscular weakness. Sections were examined for myosin adenosine triphosphatase (ATPase) after pre-incubation with both acid buffer (pH 4.37 and 4.6) and alkaline buffer (pH 10.3), for reduced nicotinamide dinucleotide diaphorase (NADHd), and for a-glycerophosphate dehydrogenase (a-GPDH). Sections were stained with hematoxilin and eosin to look for pathological changes and examined with a transmission electron microscope. Skeletal muscle of patients in early stage of sepsis showed a normal aspect with light microscopy, but at the ultrastructural level some of the fibres showed atrophy and some capillaries looked altered . Patients with sepsis/MOFS exhibited an evident muscle disorder with oedema. infiltrate, atrophy and segmental necrosis. All fibre types showed decrease in diameter; specially fibre types IIA and lIB . Intramuscular capillaries were thickened and occluded , indexes of capillarity were slightly reduced, and fibre oxidative activity was decreased. At ultrastructural level fibres showed severe atrophy, contractile system disorganization and segmental necrosis. Capillaries were also altered and the mononuclear cell infiltrate was abundant and represented by macrophages. lymphocytes and mastocytes.es
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dc.format.extent8es
dc.identifier.citationHistology and histopathology, Vol. 13, nº 1 (1998)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/92551
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectSepsises
dc.subjectMOFSes
dc.subjectSkeletal musclees
dc.subjectUltrastructurees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleHistochemical and ultrastructural study of skeletal muscle in patients with sepsis and multiple organ failure syndrome (MOFS)es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.13, nº 1 (1998)