Publication: Growth hormone and prolactin immunoreactivity in the pituitary gland of postnatal little (lit) mice
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Date
1986
Authors
Wilson, D.B. ; Wyatt, D.P.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Homozygous little (/it/lit) mutant mice exhibit
a growth lag which is manifested at approximately two
weeks postnatally. Functional aspects of the development
of piruitary growth hormone (GH) cells and prolactin
(PRL) cells were thus analyzed by means of colloidal gold
immunocytochemistry at the ultrastructural level in lit/lit
mice and their normal counterparts ranging in age from
5 days postnatally to adulthood. In the adult normal and
lit/lit pituitaries, secretory granules in GH cells and PRL
cells showed a positive immunoreaction to their respective
antisera, as did granules in both cell-types at 5 days
postnatally. By 14 days some GH cells in lit/lit pituitaries
appeared to be less densely populated with granules than
GH cells in normal pituitaries, but a positive
immunoreaction continued to occur even in sparsely
granulated GH cells. PRL cells showed ultrastructural
features in lit/lit pituitaries which were similar to those in
normal mice, and immunoreactivity was present at all
stages examined. The results indicate that since differences
in granule reactivity were not evident between lit/lit and
normal GH cells, despite ultrastructural morphologic
differences which were present by 14 days postnatally,
manifestations of the defect in lit/lit may be primarily
quantitative in terms of numbers of granules and/or
numbers of GH cells. With respect to PRL cells, neither
morphologic nor functional aberrations could be observed;
thus, a deficit in PRL hormone production might be the
result of a more subtle defect than that in GH cells.
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