Publication: Evolución de la actividad de la Bilirrubina-UDP Glucuronosil-Transferasa (bUDP-GT) durante el desarrollo en rata
Authors
Cantarino, María Helena ; Arahuetes, Rosa María ; Arza, Elvira ; Cubero, Francisco J. ; García-Barrutia, María del Socorro ; Ortiz, Agustín
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Facultad de Biología
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Publisher
Murcia: Universidad de Murcia, Servicio de Publicaciones
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DOI
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info:eu-repo/semantics/article
Description
Abstract
La enzima microsomal bUDP-GT (bilirrubina uridindifosfo-glucuronosil transferasa) es responsable de la conjugación de la bilirrubina para
ser eliminada por la bilis. Es un hecho establecido que durante la gestación la madre conjuga y elimina la bilirrubina producida por el feto, si bien hemos demostrado en nuestro laboratorio que el gen responsable de dicha enzima comienza a expresarse a partir del día 13 de gestación y por ello, nos planteamos: 1º) la puesta a punto de la téc
nica para determinar la actividad de la bUDP-GT en tejido hepático, y 2º) aportar nuevos datos sobre la variación de la actividad de la enzima durante el desarrollo perinatal. En cuanto al primer apartado, se han analizado el tiempo de activación de la fracción microsomal, que se estabiliza a los 30 minutos de incubación y la variación de la actividad enzimática cuyo máximo aparece a los 5 minutos de incubación
de la mezcla de reacción. En cuanto a la evolución de la actividad de la bUDP-GT con la edad, hemos visto que sólo al final del periodo de gestación comienzan a aparecer valores detectables y durante el primer mes de vida ya alcanzan valores próximos al adulto.
Conjugation of bilirubin and excretion by the liver is catalysed by the microsomal enzyme bilirubin-UDP-glucuronosyltransferase (bUDP-GT). It is well known that during gestation, the fetal liver in utero is incapa ble for capture, conjugation and clearance of bilirubin. However, we have previously demonstrated that the gene responsible for the enzyme starts to express by day 13 of gestation. Therefore, the aims of the present work are first, to carry out a procedure so as to determine the activity of the bUDP-GT in the hepatic tissue, and, second, to obtain new data about the changes during the perinatal development in the activity le vels of the mentioned enzyme. On the one hand, we have analyzed both, the activation time of the microsomal fraction, which stabilizes af ter 30 minutes of incubation, and the changes in the enzyme activity which reach a maximum peak after 5 minutes of incubation. On the other hand, we only detect activity of the bUDP-GT at the final period of ges tation, reaching adult levels during the first month of extrauterine life.
Conjugation of bilirubin and excretion by the liver is catalysed by the microsomal enzyme bilirubin-UDP-glucuronosyltransferase (bUDP-GT). It is well known that during gestation, the fetal liver in utero is incapa ble for capture, conjugation and clearance of bilirubin. However, we have previously demonstrated that the gene responsible for the enzyme starts to express by day 13 of gestation. Therefore, the aims of the present work are first, to carry out a procedure so as to determine the activity of the bUDP-GT in the hepatic tissue, and, second, to obtain new data about the changes during the perinatal development in the activity le vels of the mentioned enzyme. On the one hand, we have analyzed both, the activation time of the microsomal fraction, which stabilizes af ter 30 minutes of incubation, and the changes in the enzyme activity which reach a maximum peak after 5 minutes of incubation. On the other hand, we only detect activity of the bUDP-GT at the final period of ges tation, reaching adult levels during the first month of extrauterine life.
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Citation
Anales de biología, vol. 24, 2002, 155-160
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