Publication: Stromal cells and extracellular matrix
components in spontaneous canine transmissible
venereal tumour at different stages of growth
Authors
Mukaratirwa, S. ; Chimonyo, M. ; Obwolo, M. ; Gruys, E. ; Nederbragt, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Stromal cells and extracellular matrix (ECM)
components are important for tumour cell behaviour.
Little is known about the role of stromal cells and ECM
components in the progression and regression of
spontaneous canine transmissible venereal tumour
(CTVT). In this study, the stromal cell type was
determined by immunohistochemical labelling with
antibodies to desmin, vimentin and a-smooth muscle
actin (a-SMA) during the progressive and regressive
stages of spontaneous CTVT. The distribution of ECM
components tenascin-C, chondroitin sulphate and
versican were determined immunohistochemically, and
hyaluronan distribution was determined using a
biotinylated protein complex with specific affinity for
hyaluronan. Stromal cells of tumours in both the
progressive and regressive stage were positive for
vimentin and negative for desmin. The number of
stromal cells expressing a-SMA was significantly higher
(P=0.001) in regressing tumours, than progressing
tumours. These results suggest that the modulation of
stromal cells that occurs during the regression of CTVT
is similar to that occurring during wound healing.
Tenascin-C was weakly expressed in the stroma of
tumours in the progressive stage and in regions of the
regressing tumours with tumour infiltrating lymphocytes
(TILs), but intensely expressed in the stroma of tumours
in late regressive stage. In addition, tenascin-C was also
expressed in the cytoplasm of some tumour cells in the
late regressive stage. A strong stromal tenascin-C
intensity was significantly associated with regressing
tumours (P=0.001). Strong stromal hyaluronan intensity
and a high proportion of hyaluronan-positive tumour
cells were significantly associated with progressing
tumours (P=0.001). This suggests that hyaluronan is
involved in the growth of the tumour. There was no
significant difference in the expression of chondroitin sulphate and versican in progressing and regressing
tumours.
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