Publication: Biological behavior of Leishmania (L.)
amazonensis isolated from a human diffuse
cutaneous leishmaniasis in inbred strains of mice
Authors
Cupolilo, S.M.N. ; Souza, C.S.F. ; Abreu Silva, A.L. ; Calabrese, K.S. ; Gonçalves da Costa, S.C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
After a subcutaneous injection of 104
purified amastigotes of an isolate from a diffuse case of
cutaneous leishmaniasis caused by the
MHOM/BR/76/Ma-5 strain of Leishmania amazonensis,
three inbred mouse strains developed a progressive
nodular lesion, which evolved to an ulcerated lesion.
Based on these data, mice of BALB/c, C57BL/6 or
C57BL/10 could be classified as susceptible. The
majority of mice developed metastases in the footpads,
ear, tail, nose and oral mucosa. Amputation of the
members related to the primary lesion was frequent.
Experiments using the limiting dilution analysis showed
that there was no correlation between lesion and parasite
load. It has been demonstrated that these mouse strains
could be considered excellent models for mucocutaneous
leishmaniasis when infected with L. amazonensis.
Metastatic lesions caused destruction of the nasal
region with many parasitized macrophages under the
epithelial surface of the nasal mucosa. Bone destruction
occurred with an extensive inflammatory reaction
presenting macrophages heavily parasitized by
amastigotes. The parasites also spread to the periodontal
ligament and other structures of the oral cavity, which
could induce a severe inflammatory process. This study
indicates that both nasal and oral lesions in mice infected
by L. amazonensis were characterized by an
inflammatory reaction with the presence of a high
parasite load within macrophages.
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