Publication: Embryonic stem cells markers are present within rabbit atherosclerotic plaques
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Date
2008
Authors
Zulli, Anthony ; Buxton, Brian F. ; Black, M. Jane ; Hare, David L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Recent evidence suggests that smooth muscle
cells within atherosclerotic plaques originate from
vascular progenitor cells. We have previously shown that
smooth muscle cells and macrophages present within
rabbit atherosclerotic plaques are positive for factors of
the renin angiotensin and nitric oxide systems as well as
the hematopoietic stem-cell marker CD34 and the panleukocyte
marker CD45. To explore the idea that these
cells are of primitive types, immunohistochemistry was
used to identify pluripotent embryonic stem cells (ESC)
markers (Oct-4, SSEA1,3,4, TRA1-60, 81) in these
plaques and to compare these to intimal thickening.
Objective: To immunolocalise ESC markers in rabbit
aortic intimal thickening and atherosclerotic plaques.
Design: New Zealand White rabbits were fed either a
control (Con) diet, 0.5% cholesterol (Chol) or 1%
methionine (Meth) for 12 weeks. Animals were
perfusion fixed, aortae excised and processed for
paraffin. Immunohistochemistry was performed by
standard techniques. Results: Oct-4, SSEA 1, 3 and 4,
TRA-1-60 and TRA-1-81 were all present within in
atherosclerotic plaques. However, some cells were not
positive for TRA-1-60 and TRA-1-81. In fact, positive TRA-1-81 macrophages were uncommon, and positive
TRA-1-81 smooth muscle cells were rare. Intimal
thickening in Meth did not show any TRA-1-81 positive
cells Conclusions: Macrophages and smooth muscle
cells within atherosclerotic plaques express markers of
ESC. These results suggest that cells within these
plaques are primitive and might differentiate into other
types of cells.
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