Publication: Expression of activity-dependent neuroprotective protein in the brain of adult rats
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Date
2008
Authors
Gennet, N. ; Herden, C. ; Bubb, V.J. ; Quinn, J.P. ; Kipar, A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Activity-dependent neuroprotective protein
(ADNP) is a VIP-regulated gene, which is essential for
brain development. A synthetic peptide (NAP) derived
from the ADNP sequence is highly neuroprotective,
therefore it has been hypothesised that ADNP has a
similar role. ADNP contains classical transcription
factor motifs and nuclear localisation domains, but it has
also been reported to be secreted and to co-localise with
microtubules, indicating that ADNP may have multiple
functions. We investigated the pattern of ADNP
expression by immunohistology in normal rat brain, in
order to generate a framework for future studies
examining changes in ADNP expression in response to
noxious stimuli or in models of disease. We found
widespread ADNP-like immunoreactivity in neurons
throughout the rat brain, with the highest expression in
the cerebellum, and strong expression in the thalamus,
mesencephalon, pons and medulla oblongata. ADNPlike
immunoreactivity was mainly observed in the
cytoplasm of neurons, and fibre tracts were often
strongly positive as well. In addition, positive neuronal
nuclei were occasionally observed. ADNP-like immunoreactivity
was lost in degenerating ‘dark’ neurons, the morphologically unaltered adjacent cells. Occasional
astrocyte and microglial cells were also positive. We
suggest that the widespread expression of ADNP may
correlate with the wide-ranging protective effects of
NAP, and that the cytoplasmic and axonal localisation of
ADNP-like immunoreactivity suggests additional, nontranscriptional
functions of ADNP
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