Publication: Reduced UVB-induced corneal damage caused by
reactive oxygen and nitrogen species and decreased
changes in corneal optics after trehalose treatment
Authors
Cejkova, Jitka ; Cejka, Čestmír ; Ardan, T. ; Širc, Jakub ; Michálek, Jiří ; Luyckx, Jacques
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Trehalose, a nonreducing disaccharide of
glucose, produced and stored in many lower and higher
organisms, although not in mammals, is synthetized as a
stress responsive factor when cells are exposed to
various environmental stress conditions. Recently,
trehalose has been implicated in various situations in
mammals. The aim of this paper was to examine whether
trehalose might decrease the damage of the rabbit cornea
evoked by UVB rays. During irradiation with UVB rays,
consisiting of a daily dose of 0.5 J/cm2 for four days,
trehalose was applied in eye drops on the right eye and
buffered saline on the left eye. One day after the end of
irradiation the animals were sacrificed and the corneas
examined spectrophotometrically for light absorption.
Another group of corneas similarly treated were
examined morphologically and immunohistochemically.
Corneal thickness (hydration) was measured using a
Pachymeter. The results show that compared to buffered
saline, trehalose treated corneas displayed fewer corneal
disturbances during UVB irradiation. The increases in
corneal hydration and light absorption were less
pronounced and intracorneal inflammation and corneal
neovascularization were suppressed. Nitric oxide
synthases that generate nitric oxide were less expressed
in the cornea, and formation of cytotoxic peroxynitrite
(demonstrated by nitrotyrosine residues) was decreased.
The expression of the antioxidant aldehyde
dehydrogenase3A1 was less inhibited in the corneal
epithelium, and apoptotic corneal epithelial cell death (detected by immunostaining for active caspase-3) was
greatly diminished. In conclusion, trehalose reduced
UVB-induced damage caused by reactive oxygen and
nitrogen species and decreased changes in the corneal
optics.
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