Publication: Gonadotrophin-releasing hormone immunoreactivity in the brain of the tropical freshwater fish, Pygocentrus notatus (Teleostei-Characidae)
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Date
1993
Authors
Cáceres-Dittmar, G. ; Tapia, F.J. ; Guerrero, H. Y. ; Paiva, C. L. ; Marcano, D.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The distribution of GnRH in the brain of the
teleost Pygocentrus notatus was demonstrated with the
avidin-biotin peroxidase immunocytochemical method
using highly specific antibody against synthetic
mammalian GnRH. Optimal immunoreaction was
obtained using: 1) Bouin's fluid for fixation; 2) repeated
incubation with primary antiserum; 3) the use of a
detergent in the dilution buffer; 4) the high sensitivity of
the avidin-biotin immunoperoxidase method with the
cobalt intensification of 3-3'diaminobenzidine
tetrahydrochloride; and 5) the use of primary antibody
with high specificity. GnRH-immunoreactive (GnRH-ir)
in cells andlor axons was observed in al1 main brain
regions. In the forebrain, GnRH-ir was located in a
network extending from the caudal part of the olfactory
bulb to the telencephalon. GnRH-ir fibres were also
observed in the optic tectum, cerebellum and
hypothalamus. Two groups of neurona1 cell bodies were
identified. One group was located in the antero-ventral
telencephalon corresponding to the nucleus olfactoretinalis.
The second group was found in the rostrodorsal
hypothalamus. No GnRH-ir material was detected in the
pituitary gland, thus confirming the results of previous
studies on brain GnRH-ir distribution obtained by
radioimmunoanalysis in this species. These results
demonstrate a high degree of similarity between the
GnRH systems of P. notatus and other teleost species.
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